Reference Report

Reference
Citation	Adams, M.D., Tarng, R.S., Rio, D.C. (1997). The alternative splicing factor PSI regulates P-element third intron splicing in vivo. Genes Dev. 11(1): 129--138. (Export to RIS)
FlyBase ID	FBrf0091004
Publication Type	Research paper
PubMed ID	9000056
PubMed Abstract	Splicing of the Drosophila P-element third intron (IVS3) is inhibited in somatic cells, restricting transposase expression to the germ line. Somatic inhibition of IVS3 splicing involves the assembly of a multiprotein complex on a regulatory sequence in the IVS3 5' exon. The P-element somatic inhibitor protein (PSI) is a component of this ribonucleoprotein complex and is required for inhibition of IVS3 splicing in vitro. The soma-specific expression pattern of PSI suggests that its low abundance in the germ line allows IVS3 splicing. We demonstrate that ectopic expression of PSI in the female germ line is sufficient to repress splicing of an IVS3 reporter transgene. We also show that IVS3 splicing is activated in somatic embryonic cells in the presence of an antisense PSI ribozyme. These results support the model that PSI is a tissue-specific regulator of IVS3 splicing in vivo.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (7)
	LTSV\RBZ^Psi.hs P\T^{IVS3.hs.T:Ecol\lacZ} P\T^{IVS3.Hsp83.T:Ecol\lacZ} P\T^{Δ2-3.Hsp83.T:Ecol\lacZ} Psi^Hsp83.PA w^+mC w^+mW.hs
Constructs (5)
	P{hsp70-IVS3-βgeo} P{hsp70-PSI.ribozyme} P{hsp83-IVS3-βgeo} P{hsp83-PSI} P{hsp83-Δ2-3-βgeo}
Genes (5)
	LTSV\RBZ P\T Psi T:Ecol\lacZ w
Natural transposons (1)
	P-element