Reference Report

Reference
Citation	Golic, K.G., Golic, M.M. (1996). Engineering the Drosophila genome: chromosome rearrangements by design. Genetics 144(4): 1693--1711. (Export to RIS)
FlyBase ID	FBrf0091061
Publication Type	Research paper
PubMed ID	8978056
PubMed Abstract	We show that site-specific recombination can be used to engineer chromosome rearrangements in Drosophila melanogaster. The FLP site-specific recombinase acts on chromosomal target sites located within specially constructed P elements to provide an easy screen for the recovery of rearrangements with breakpoints that can be chosen in advance. Paracentric and pericentric inversions are easily recovered when two elements lie in the same chromosome in opposite orientation. These inversions are readily reversible. Duplications and deficiencies can be recovered by recombination between two elements that lie in the same orientation on the same chromosome or on homologues. We observe that the frequency of recombination between FRTs at ectopic locations decreases as the distance that separates those FRTs increases. We also describe methods to determine the absolute orientation of these P elements within the chromosome. The ability to produce chromosome rearrangements precisely between preselected sites provides a powerful new tool for investigations into the relationships between chromosome arrangement, structure, and function.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (8)
	Df(2R)RS3r-4A+RS5r-7A Dp(2;2)RS3r-4A+RS5r-7A In(2LR)RS5r-3A+RS3r-4A In(2LR)RS5r-3B+RS3r-4A In(2LR)RS5r-8+RS3r-4A In(2R)RS3r-4A+RS5r-31B In(3L)RS5r-1A+RS3r-2 In(3LR)RS3r-2+RS5r-2A
Alleles (8)
	l(2)51Fb^4A ry^+t7.2 Scer\FLP1^hs.PP w^+mW.hs w^{+mW.Scer\FRT.hs3} w^{+mW.Scer\FRT.hs} w^RS3r w^RS5r.hs
Constructs (28)
	001001 001002 001003 001378 001379 001380 001381 001382 001383 ^EcoRIef/^HindIIIef Car1_P3'(H3,P3') Car1_P5'(P5',R1) FRT_200bp Hsp70A_P370(Ha3,Xm) P{3'.RS5+3.3'} P{5'.1xFRT.RS.5'} P{70FLP} P{RS3} P{RS3r} P{RS5} P{RS5r} pP{70FLP} pP{RS3} pP{RS5} RS3.RS5.mW.FRT w⁺0.46(Ma1,Bcl1) w⁺0.067(Bcl1,H3) w⁺3.7(Xb,R1)
Genes (4)
	l(2)51Fb M(2)53 Scer\FLP1 w
Insertions (33)
	P{3'.RS5+3.3'}1A,2 P{3'.RS5+3.3'}2A,2 P{3'.RS5+3.3'}3A,4A P{3'.RS5+3.3'}3B,4A P{3'.RS5+3.3'}7A,4A P{3'.RS5+3.3'}8,4A P{3'.RS5+3.3'}31B,4A P{5'.1xFRT.RS.5'}7A,4A P{70FLP}3F P{RS3}2 P{RS3}3 P{RS3}l(2)51Fb^4A P{RS3r}2 P{RS3r}3 P{RS3r}4A P{RS5}1A P{RS5}2A P{RS5}3A P{RS5}3B P{RS5}7A P{RS5}8 P{RS5}10 P{RS5}15B P{RS5}31B P{RS5r}1A P{RS5r}2A P{RS5r}3A P{RS5r}3B P{RS5r}7A P{RS5r}8 P{RS5r}10 P{RS5r}15B P{RS5r}31B
Libraries/Collections (1)
	DrosDel_TI_Df_datasets