Reference Report

Reference
Citation	Hagstrom, K., Muller, M., Schedl, P. (1996). Fab-7 functions as a chromatin domain boundary to ensure proper segment specification by the Drosophila bithorax complex. Genes Dev. 10(24): 3202--3215. (Export to RIS)
FlyBase ID	FBrf0091065
Publication Type	Research paper
PubMed ID	8985188
PubMed Abstract	Fab-7 deletions in the bithorax complex have a novel gain-of-function phenotype, typically transforming parasegment 11 (PS11) into PS12 identity. Genetic analysis indicates that removal of the Fab-7 element results in the fusion of the iab-6 (PS11) and iab-7 (PS12) cis-regulatory domains into a single regulatory domain that inappropriately regulates Abdominal-B in PS11. This has led to the hypothesis that Fab-7 is a chromatin domain boundary that normally functions to ensure the autonomous activity of the iab-6 and iab-7 cis-regulatory domains. We use several different enhancer blocking assays to demonstrate that Fab-7 has the insulating properties expected of a domain boundary. We define a minimal fragment of Fab-7 sufficient for enhancer blocking, and demonstrate that it is completely distinct from an adjacent Polycomb-dependent silencer. We compare Fab-7 to the su(Hw) insulator element, and show that Fab-7 enhancer blocking activity is intermediate between that of five and twelve reiterated binding sites for the Su(Hw) protein. These results support the model that Fab-7 functions as a domain boundary within the context of the bithorax complex, making Fab-7 one of the first boundary elements that is known to have an essential function in vivo.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (38)
	Abd-B^blt Abd-B^Fab7-1 Abd-B^Fab7-2 Abd-B^Fab7-3 Ecol\lacZ^{ftz.Fab-7.1.7.Hsp70Bb} Ecol\lacZ^{ftz.Fab-7.1.7} Ecol\lacZ^ftz.ff#1 Ecol\lacZ^{ftz.random.Hsp70Bb} Ecol\lacZ^{ftz.su(Hw)5.Hsp70Bb} Ecol\lacZ^{ftz.su(Hw)12.Hsp70Bb} Ecol\lacZ^ftz.su(Hw)12 Ecol\lacZ^Hsp70Bb.-194 Ecol\lacZ^Hsp70Bb.ftz Fab-7^1.7 Fab-7^3.35 Fab-7^HA Fab-7^HN Fab-7^NE Fab-7^PA gypsy\su(Hw)BR¹⁶⁰ gypsy\su(Hw)BR³⁶⁷ iab-7PRE^AX mod(mdg4)^ul scs'^0.5 su(Hw)⁷ su(Hw)^V w^+mC w^{enh.Fab-7.1.7} w^enh.Fab-7.4 w^enh.Fab-7.HA w^enh.Fab-7.HN w^enh.Fab-7.NE w^enh.Fab-7.PA w^{enh.iab-7PRE.AX} w^enh.random w^enh w^Fab-7.5.enh w^RW
Constructs (21)
	P{Fab7-ff#2} P{Fab7-fh#2} P{Fab7-w#4} P{Fab7-w#5} P{Fab7-w#6} P{Fab7-w#7} P{Fab7-w#8} P{Fab7-w#9} P{Fab7-w#10} P{Fab7-w#11} P{ftz/lacC} P{ftz-lacZ.ff#1} P{Hsp70-lacZ-fh#0} P{Hsp70-lacZ-fh#1} P{iab-7PRE-w.AX} P{su(Hw)BR-ff#3} P{su(Hw)BR-fh#3} P{su(Hw)BR-fh#4} P{w^RW} P{w^RW+we2} P{w^RW+we}
Genes (9)
	Abd-B Ecol\lacZ Fab-7 gypsy\su(Hw)BR iab-7PRE mod(mdg4) scs' su(Hw) w
Insertions (1)
	P{bluetail}Abd-B^blt