Reference Report

Reference
Citation	Preston, C.R., Sved, J.A., Engels, W.R. (1996). Flanking duplications and deletions associated with P-induced male recombination in Drosophila. Genetics 144(4): 1623--1638. (Export to RIS)
FlyBase ID	FBrf0091145
Publication Type	Research paper
PubMed ID	8978050
PubMed Abstract	We studied P element-induced recombination in germline mitotic cells by examining the structure of the recombinant chromosomes. We found that most recombinants retain a mobile P element at the site of the recombination, usually with either a deletion or a duplication immediately adjacent to the P end at which the crossover occurred. The sizes of these deletions and duplications ranged from a few base pairs to well over 100 kb. These structures fit the "hybrid element insertion" (HEI) model of male recombination in which the two P-element copies on sister chromatids combine to form a "hybrid element" whose termini insert into a nearby position on the homologue. The data suggest that P-induced recombination can be used as an efficient means of generating flanking deletions in the vicinity of existing P elements. These deletions are easily screened using distant flanking markers, and they can be chosen to extend in a given direction depending on which reciprocal recombinant type is selected. Furthermore, the retention of a mobile P element allows one to extend the deletion or generate additional variability at the site by subsequent rounds of recombination.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (25)
	Df(2R)50C-16 Df(2R)50C-21 Df(2R)50C-36 Df(2R)50C-38 Df(2R)50C-40 Df(2R)50C-43 Df(2R)50C-45 Df(2R)50C-49 Df(2R)50C-68 Df(2R)50C-69 Df(2R)50C-71 Df(2R)50C-81 Df(2R)50C-85 Df(2R)50C-89 Df(2R)50C-101 Df(2R)50C-102 Df(2R)50C-107 Df(2R)50C-111 Df(2R)50C-116 Df(2R)50C-128 Df(2R)50C-129 Df(2R)50C-131 Df(2R)50C-132 Df(2R)50C-133 Df(2R)mam⁰⁴⁶¹⁵
Alleles (5)
	AGO1⁰⁴⁸⁴⁵ fas⁰⁵⁴⁸⁸ l(2)00248⁰⁰²⁴⁸ mam⁰⁴⁶¹⁵ w^+mC
Constructs (1)
	P{CaSpeR}
Genes (9)
	AGO1 Cp1 fas l(2)50CDa l(2)00248 mam ms(2)50Ca pea w
Insertions (2)
	P{CaSpeR}Cp1^50C P{PZ}mam⁰⁴⁶¹⁵
Natural transposons (1)
	P-element