Reference Report

Reference
Citation	Schnorr, J.D., Berg, C.A. (1996). Differential activity of Ras1 during patterning of the Drosophila dorsoventral axis. Genetics 144(4): 1545--1557. (Export to RIS)
FlyBase ID	FBrf0091163
Publication Type	Research paper
PubMed ID	8978043
PubMed Abstract	In Drosophila, the Ras1 gene is required downstream of receptor tyrosine kinases for correct eye development, embryonic patterning, wing vein formation, and border cell migration. Here we characterize a P-element allele of Ras1, Ras1(5703), that affects viability, eye morphogenesis, and early and late stages of oogenesis. Flies transheterozgyous for Ras1(5703) and existing EMS-induced Ras1 alleles are viable and exhibit a range of eye and eggshell defects. Differences in the severity of these phenotypes in different tissues suggest that there are allele-specific effects of Ras1 in development. Analysis of rescue constructs demonstrates that these differential phenotypes are due to loss of function in Ras1 alone and not due to effects on neighboring genes. Females mutant at the Ras1 locus lay eggs with reduced or missing dorsal eggshell structures. We observe dominant interactions between Ras1 mutants and other dorsoventral pathway mutants, including Egfr(top) and gurken. Ras1 is also epistatic to K10. Unlike Egfr(top) and gurken mutants, however, Ras1 females are moderately fertile, laying eggs with ventralized eggshells that can hatch normal larvae. These results suggest that Ras1 may have a different requirement in the patterning of the eggshell axis than in the patterning of the embryonic axis during oogenesis.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (4)
	Df(2R)Egfr18 Df(3R)by10 Df(3R)ΔC17b Df(3R)ΔC40b
Alleles (18)
	Egfr^top-18A fs(1)K10¹ grk³ mRpL47^tSa Ras85D⁰⁵⁷⁰³ Ras85D^+t5.5 Ras85D^+t12 Ras85D^e1B Ras85D^e2F Ras85D^ix12a Ras85D^ix13b Ras85D^Sutor-341 Ras85D^Sutor-404 Ras85D^Δ Ras85D^ΔC17b Rlb1^hs.PE Rlb1^tSa Rlb1^Δ
Constructs (10)
	P{HSP-Rlb1} P{pR5.5} P{pR5.5ΔRas1} P{pR5.5ΔRlb1} P{PZ} P{PZ-Δix12a} P{PZ-Δix13b} P{R5.5} P{R5.5ΔRas1} P{R5.5ΔRlb1}
Genes (6)
	Egfr fs(1)K10 grk mRpL47 Ras85D Rlb1
Insertions (3)
	P{PZ}Ras85D⁰⁵⁷⁰³ P{PZ-Δix12a}Ras85D^ix12a P{PZ-Δix13b}Ras85D^ix13b
Natural transposons (1)
	P-element