Reference Report

Reference
Citation	Schulze, K.L., Bellen, H.J. (1996). Drosophila syntaxin is required for cell viability and may function in membrane formation and stabilization. Genetics 144(4): 1713--1724. (Export to RIS)
FlyBase ID	FBrf0091165
Publication Type	Research paper
PubMed ID	8978057
PubMed Abstract	The role of the Drosophila homologue of syntaxin-1A (syx) in neurotransmission has been extensively studied. However, developmental Northern analyses and in situ hybridization experiments show that SYX mRNA is expressed during all stages and in many tissues. We have isolated new mutations in syx that reveal roles for syx outside the nervous system. In the ovary, SYX is present in the germarium, but it is predominantly localized to nurse cell membranes. Mitotic recombination experiments in the germline show SYX is essential for oogenesis and may participate in membrane biogenesis in the nurse cells. In the early embryo, a large contribution of maternally deposited RNA is present, and the protein is localized at cell membranes during cellularization. After the maternal contribution is depleted, zygotically produced SYX assists secretion events occurring late in embryogenesis, such as cuticle deposition and neurotransmitter release. However, SYX is also required in larval imaginal discs, as certain hypomorphic mutant combinations exhibit rough eyes and wing notch defects indicative of cell death. Furthermore, recombinant clones that lack syx cause cell lethality in the developing eye. We propose that, similar to its roles in cuticle secretion and neurotransmitter release, SYX may mediate membrane assembly events throughout Drosophila development.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (3)
	Df(3R)crb-F89-4 In(2LR)O In(3LR)TM6B
Alleles (20)
	Ecol\lacZ^abd-A.P Syx1A¹ Syx1A² Syx1A⁴ Syx1A¹⁴ Syx1A^15ts Syx1A¹⁷ Syx1A⁸⁶ Syx1A¹¹⁸ Syx1A¹⁷⁷ Syx1A²⁰⁴ Syx1A²⁸⁴ Syx1A²⁸⁷ Syx1A²⁹⁴ Syx1A⁰⁶⁷³⁷ Syx1A^+t6 Syx1A^+t11 Syx1A^Δ6 Syx1A^Δ229 w^+mC
Balancers (2)
	CyO-509 TM6B,abdA-LacZ
Constructs (12)
	P{AbdA-lacZ} P{iab-2(1.7)lacZ} P{PZ} P{PZ-Δ.s2} P{PZ-Δ.s4} P{PZ-Δ.s17} P{PZ-Δ.s118} P{PZ-Δ.s177} P{PZ-Δ.s287} P{PZ-Δ.s294} P{syx⁺6} P{syx⁺11}
Genes (4)
	Ecol\lacZ Fas2 Syx1A w
Insertions (10)
	P{5'3'}Syx1A²⁰⁴ P{iab-2(1.7)lacZ}6B P{PZ}Syx1A⁰⁶⁷³⁷ P{PZ-Δ.s2}Syx1A² P{PZ-Δ.s4}Syx1A⁴ P{PZ-Δ.s17}Syx1A¹⁷ P{PZ-Δ.s118}Syx1A¹¹⁸ P{PZ-Δ.s177}Syx1A¹⁷⁷ P{PZ-Δ.s287}Syx1A²⁸⁷ P{PZ-Δ.s294}Syx1A²⁹⁴