Reference Report

Reference
Citation	Clark, I.E., Jan, L.Y., Jan, Y.N. (1997). Reciprocal localization of Nod and kinesin fusion proteins indicates microtubule polarity in the Drosophila oocyte, epithelium, neuron and muscle. Development 124(2): 461--470. (Export to RIS)
FlyBase ID	FBrf0092501
Publication Type	Research paper
PubMed ID	9053322
PubMed Abstract	Polarization of the microtubule cytoskeleton is an early event in establishment of anterior-posterior polarity for the Drosophila oocyte. During stages 8-9 of oogenesis, when oskar mRNA is transported to the posterior pole of the oocyte, a fusion protein consisting of the plus-end-directed microtubule motor kinesin and beta-galactosidase (Kin:beta gal) similarly localizes to the posterior pole, thereby suggesting that plus ends of microtubules are pointed to the posterior. In this paper, we have substituted the motor domain of Kin:beta gal with the putative motor domain (head) from the kinesin-related protein Nod. In cells with defined microtubule polarity, the Nod:beta gal fusion protein is an in vivo minus-end reporter for microtubules. Nod:beta gal localizes to apical cytoplasm in epithelial cells and to the poles of mitotic spindles in dividing cells. In stage 8-10 oocytes, the Nod fusion localizes to the anterior margin, thus supporting the hypothesis that minus ends of microtubules at these stages are primarily at the anterior margin of the oocyte. The fusion protein also suggests a polarity to the microtubule cytoskeleton of dendrites and muscle fibers, as it accumulates at the ends of dendrites in the embryonic PNS and is excluded from terminal cytoplasm in embryonic muscle. Finally, the reciprocal in vivo localization of Nod:beta gal and Kin:beta gal suggests that the head of Nod may be a minus-end-directed motor.
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Secondary IDs	FBrf0092826
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (14)
	Ecol\lacZ^KZ503 Ecol\lacZ^KZ1071 Ecol\lacZ^{P\T.T:Khc-606} Khc::nod^118.3 Khc::nod^133.4 Khc::nod^143.2 Khc::nod^153.1 Khc::nod^{Scer\UAS.T:Ecol\lacZ} Khc::nod^T:Ecol\lacZ Khc^{Scer\UAS.T:Ecol\lacZ} Scer\GAL4^elav.PLu Scer\GAL4^how-24B Scer\GAL4^hs.PB w^+mC
Constructs (6)
	P{KZ.TRAP} P{NZ} P{UAS-Khc::lacZ} P{UAS-Khc::nod::lacZ} pP{NZ} pP{UAS-Khc::nod::lacZ}
Genes (7)
	Ecol\lacZ Khc Khc::nod nod Scer\GAL4 T:Ecol\lacZ w
Insertions (8)
	P{KZ.TRAP}KZ503 P{KZ.TRAP}KZ1071 P{NZ}118.3 P{NZ}133.4 P{NZ}143.2 P{NZ}153.1 P{UAS-Khc::lacZ}30 P{UAS-Khc::lacZ}31Y