Reference Report

Reference
Citation	Hepker, J., Wang, Q.T., Motzny, C.K., Holmgren, R., Orenic, T.V. (1997). Drosophila cubitus interruptus forms a negative feedback loop with patched and regulates expression of Hedgehog target genes. Development 124(2): 549--558. (Export to RIS)
FlyBase ID	FBrf0092552
Publication Type	Research paper
PubMed ID	9053330
PubMed Abstract	The Drosophila segment polarity gene cubitus interruptus (ci) encodes a zinc finger protein that is required for the proper patterning of segments and imaginal discs. Epistasis analysis indicates that ci functions in the Hedgehog (Hh) signal transduction pathway and is required to maintain wingless expression in the embryo. In this paper, the role of the Ci protein in the Hh signaling pathway is examined in more detail. Our results show that ectopic expression of ci in imaginal discs and the embryo activates the expression of Hh target genes. One of these target genes, patched, forms a negative feedback loop with ci that is regulated by Hh signal transduction. Activation is also achieved using the Ci zinc finger domain fused to a heterologous transactivation domain. Conversely, repression of Hh target genes occurs in animals expressing the Ci zinc finger domain fused to a repression domain. To examine Ci function in more detail, regions of the Ci protein that are responsible for its ability to transactivate and its subcellular distribution have been identified.
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Secondary IDs	FBrf0092840
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (26)
	ci::en^{Zn:RD.Scer\UAS.T:Hsap\MYC} ci^Act5C.PH ci^Ce-1 ci^{N:Zn.Scer\UAS.T:Ivir\HA1} ci^{N:Zn.Scer\UAS} ci^{Scer\FRT.Act5C} ci^Scer\UAS.cHa ci^Scer\UAS.cHb ci^{Zn:AD.Scer\UAS.T:Hsap\MYC,T:Scer\GAL4} ci^{Zn:C.Scer\UAS} ci^{Zn.Scer\UAS.T:Hsap\MYC} Ecol\lacZ^dpp-10638 Ecol\lacZ^en-Xho25 Ecol\lacZ^hh-P30 Ecol\lacZ^ptc.PS Ecol\lacZ^wg-en11 hh⁴ Hsp70Bb^polyA ptc⁹ ptc^G20 ry^+t7.2 Scer\GAL4^71B Scer\GAL4^en-e16E Scer\GAL4^prd.RG1 Scer\GAL4^ptc-559.1 w^+mC
Constructs (12)
	P{Act5C(FRT.polyA)ci.H} P{Act5C(-FRT)ci.H} P{GAL4-prd.F} P{ptc-lacZ.S} P{UAS-ci-cDNA} P{UAS-ci-genomic} P{UAS-ciN/Zn} P{UAS-ciN[HA]/Zn} P{UAS-ciZn/C} P{UAS-ciZn/EnRD} P{UAS-ciZn/GAL4AD} P{UAS-ciZn}
Genes (15)
	ci ci::en dpp Ecol\lacZ en hh Hsp70Bb ptc ry Scer\GAL4 T:Hsap\MYC T:Ivir\HA1 T:Scer\GAL4 w wg
Insertions (7)
	P{en1}wg^en11 P{en2.4-GAL4}e16E P{en-lacZ(Xho)}en^Xho25 P{GawB}71B P{GawB}ptc^559.1 P{PZ}dpp¹⁰⁶³⁸ P{PZ}hh^P30