A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Reference Report

Reference
Citation Ye, X., Fong, P., Iizuka, N., Choate, D., Cavener, D.R. (1997). Ultrabithorax and Antennapedia 5' untranslated regions promote developmentally regulated internal translation initiation.  Mol. Cell. Biol. 17(3): 1714--1721. (Export to RIS)
FlyBase ID FBrf0092733
Publication Type Research paper
PubMed ID 9032298
PubMed Abstract The 5' untranslated regions (UTRs) of the Drosophila Ubx and Antp genes were tested for their ability to promote cap-independent translation initiation. The Ubx and the Antp 5' UTR were inserted between the CAT and lacZ coding sequences in a dicistronic gene and tested for IRES activity in transgenic Drosophila. Northern analysis of the mRNAs showed the presence of the predicted full-length dicistronic mRNAs. High CAT activity was expressed from the first cistron from all of the dicistronic constructs introduced into the fly genome. The dicistronic transgenic strains bearing the Ubx and Antp IRES elements expressed significant levels of beta-galactosidase (betaGAL) from the second cistron whereas little or no betaGAL was expressed in the controls lacking the IRESs. In situ analysis of betaGAL expression in the transgenic strains indicates that expression of the second cistron is spatially and temporally regulated. Although the developmental patterns of expression directed by the Antp and Ubx IRESs overlap, they exhibit several differences indicating that these IRESs are not functionally equivalent.
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Secondary IDs FBrf0092874
Language of Publication English
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Publication Type Journal
Abbreviation Mol. Cell. Biol.
Title Molecular and Cellular Biology
Publication Year 1981-
ISBN/ISSN 0270-7306
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