Subject: FlyBase query Dear Dr. Hiromi, I am curating your paper: Butler et al., 1997, Development, 124: 781--792 for FlyBase. FlyBase currently has the name of the gene that the H214 insertion is located in as h214. This name will be changed to klingon (klg). I am writing to ask you what the relationship between klingon and the gene E(sev)3D is. In your abstract from the 35th Annual Drosophila Research Conference 1994 (page 231) you say that h214 (i.e. klingon) and E(sev)3D may be identical genes, since recessive lethal mutations of h214 do not complement a recessive lethal allele of E(sev)3D. I would be grateful if you could tell me whether klingon and E(sev)3D are the same gene or whether they are in fact two separate genes ? I look forward to hearing from you, Gillian Millburn \-------------------------------------------------------------- Gillian Millburn. FlyBase (Cambridge), \-------------------------------------------------------------- > Subject: FlyBase query Dear Dr. Hiromi, I have another question about your paper: Butler et al., 1997, Development, 124: 781--792 In the paper you use S2 cells that express a Neuroglian construct (S2-Nrg cells) and you reference the paper: Hortsch et al., 1995, J. Biol. Chem. 270(32): 18809--18817 Three Neuroglian constructs are mentioned in the above paper. They are: 1) A construct in which the Neuroglian 167 splice variant is expressed under the control of a metallothionein promoter. 2) A construct in which the Neuroglian 180 splice variant is expressed under the control of a metallothionein promoter. 3) A construct in which Neuroglian sequences are fused to Fasciclin 1 sequences and expressed under the control of a metallothionein promoter. I would be grateful if you could tell me which construct was in the cells you used in your paper, Gillian Millburn \-------------------------------------------------------------- Gillian Millburn. FlyBase (Cambridge), \-------------------------------------------------------------- > Subject: Re: FlyBase query Dear Dr. Millburn, Thank you very much for your mails regarding our paper: Butler et al., 1997, Development, 124: 781--792 (1) The relationship between klingon and the gene E(sev)3D. >I would be grateful if you could tell me whether klingon and E(sev)3D are t >he same gene or whether > they are in fact two separate genes ? A short answer to your query is that they are two separate genes. As is described in the 35th Annual Drosophila Research Conference 1994 (page 231), we had postulated that h214 (i.e. klingon) and E(sev)3D may be identic al genes based on the following observations: (1) The E(sev)3De0Q mutation enhances the R7-loss phenotype of a hypomorph ic allele of sevenless (sevB4). Ref. Simon et al. (1991). (2) The H214 enhancer trap strain and the klingon gene are expressed in the R7 neuron. (3) Deletions that remove part or all of the klingon gene fails to compleme nt the lethality of the E(sev)3De0Q chromosome (the screen done by Mike Si mon identified only one allele of this locus). However, we later found out that the lethality of E(sev)3De0Q (with respe ct to the failure to complement klingon mutations) can be separated from the enhancement of the sevB4 phenotype. Thus the E(sev)3De0Q chromosome con tains two unrelated hits, one that is uncovered by the deletion of the kling on gene (which we mapped to 3-78.1), and the other hit maps to the E(sev)3D locus (3-97.3). I would like to have the previous Flybase information deleted, or corrected citing the above mentioned recombination data (S.J. Butler and Y. Hiromi, unpublished, or S. J. Butler (1996), Ph. D. Thesis, Princeton University). (2) S2 cells that express a Neuroglian construct (S2-Nrg cells) The construct used in our work is: 2) A construct in which the Neuroglian 180 splice variant is expressed under the control of a metallothionein promoter. This variant is also called the 'neuron specific form'. We should have spec ified it clearly in our paper. Sincerely yours, Yasushi Hiromi ____________________________________________ At 10:06 PM 97.3.4, Gillian Millburn (Genetics) wrote: > Dear Dr. Hiromi, > > I am curating your paper: > > Butler et al., 1997, Development, 124: 781--792 > > for FlyBase. > > FlyBase currently has the name of the gene that the H214 insertion is > located in as h214. This name will be changed to klingon (klg). > > I am writing to ask you what the relationship between klingon and the gene > E(sev)3D is. In your abstract from the 35th Annual Drosophila Research > Conference 1994 (page 231) you say that h214 (i.e. klingon) and E(sev)3D > may be identical genes, since recessive lethal mutations of h214 do not > complement a recessive lethal allele of E(sev)3D. I would be grateful if > you could tell me whether klingon and E(sev)3D are the same gene or whether > they are in fact two separate genes ? > > I look forward to hearing from you, > > Gillian Millburn > I have another question about your paper: > > Butler et al., 1997, Development, 124: 781--792 > > In the paper you use S2 cells that express a Neuroglian construct (S2-Nrg > cells) and you reference the paper: > > Hortsch et al., 1995, J. Biol. Chem. 270(32): 18809--18817 > > Three Neuroglian constructs are mentioned in the above paper. They are: > > 1) A construct in which the Neuroglian 167 splice variant is expressed > under the control of a metallothionein promoter. > > 2) A construct in which the Neuroglian 180 splice variant is expressed > under the control of a metallothionein promoter. > > 3) A construct in which Neuroglian sequences are fused to Fasciclin 1 > sequences and expressed under the control of a metallothionein promoter. > > I would be grateful if you could tell me which construct was in the cells > you used in your paper, ____________________________________________________________ Yasushi Hiromi National Institute of Genetics 1111 Yata, Mishima Shizuoka 411, JAPAN > Subject: Re: FlyBase query Dear Dr. Hiromi, thankyou for your e-mail, it was very helpful. I will curate the information in your e-mail about klingon and E(sev)3D as a personal communication from you and S.J. Butler, which will correct the information we currently have in FlyBase. The changes may not be visible on the web pages immediately, as the database is updated periodically. Thankyou once again for your help, Gillian Millburn
