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Reference Report
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| Citation | Gomez, S.P., Handler, A.M. (1997). A Drosophila melanogaster hobo-white+ vector mediates low frequency gene transfer in D. virilis with full interspecific white+ complementation. Insect Mol. Biol. 6(2): 165--171. (Export to RIS) | ||
| FlyBase ID | FBrf0093108 | ||
| Publication Type | Research paper | ||
| PubMed ID | 9099580 | ||
| PubMed Abstract | Transformation of a Drosophila virilis white mutant host strain was attempted using a hobo vector containing the D. melanogaster mini-white(+) cassette (H[w(+), hawN]) and an unmodified or heat shock regulated hobo transposase helper. Two transformant lines were recovered with the unmodified helper (HFL1), one containing only the white(+) marked vector, and a sibling line containing the vector as well as an HFL1 helper integration. An approximate total transformation frequency of 1% is deduced. A high frequency of wing and eye morphology mutants were also observed, suggesting that hobo may have mobilized a related element in D. virilis. The data reaffirms a relatively low transformation vector activity for the hobo transposon in D. virilis; however, nearly full interspecific expression white(+) marker supports its possible function in other species as well. | ||
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| Language of Publication | English | ||
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Parent Publication
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| Publication Type | Compendium | ||
| Abbreviation | Insect Mol. Biol. | ||
| Title | Insect Molecular Biology | ||
| Publication Year | 1992- | ||
| ISBN/ISSN | 0307-6975 | ||
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Data from Reference
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| Alleles (4)
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| Constructs (3)
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| Genes (3)
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| Natural transposons (1)
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