|Citation||Gomez, S.P., Handler, A.M. (1997). A Drosophila melanogaster hobo-white+ vector mediates low frequency gene transfer in D. virilis with full interspecific white+ complementation. Insect Mol. Biol. 6(2): 165--171. (Export to RIS)|
|Publication Type||Research paper|
|PubMed Abstract||Transformation of a Drosophila virilis white mutant host strain was attempted using a hobo vector containing the D. melanogaster mini-white(+) cassette (H[w(+), hawN]) and an unmodified or heat shock regulated hobo transposase helper. Two transformant lines were recovered with the unmodified helper (HFL1), one containing only the white(+) marked vector, and a sibling line containing the vector as well as an HFL1 helper integration. An approximate total transformation frequency of 1% is deduced. A high frequency of wing and eye morphology mutants were also observed, suggesting that hobo may have mobilized a related element in D. virilis. The data reaffirms a relatively low transformation vector activity for the hobo transposon in D. virilis; however, nearly full interspecific expression white(+) marker supports its possible function in other species as well.|
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|Language of Publication||English|
|Additional Languages of Abstract|
|Also Published As|
|Abbreviation||Insect Mol. Biol.|
|Title||Insect Molecular Biology|
|Data from Reference|
|Natural transposons (1)|