Reference Report

Reference
Citation	Riese, J., Yu, X., Munnerlyn, A., Eresh, S., Hsu, S.C., Grosschedl, R., Bienz, M. (1997). LEF-1, a nuclear factor coordinating signaling inputs from wingless and decapentaplegic. Cell 88(6): 777--787. (Export to RIS)
FlyBase ID	FBrf0093200
Publication Type	Research paper
PubMed ID	9118221
PubMed Abstract	wingless and decapentaplegic signal during endoderm induction in Drosophila to regulate expression of the homeotic gene Ultrabithorax. Here, we define a minimal wingless response sequence in the midgut enhancer of Ultrabithorax. We show that this sequence is recognized by the murine transcription factor LEF-1 (lymphocyte enhancer binding factor 1) in a ternary complex with armadillo protein, the cytoplasmic target of the wingless signaling pathway. In stable transformants, transcriptional stimulation of the Ultrabithorax enhancer by LEF-1 depends on armadillo. Furthermore, overexpression of LEF-1 bypasses the need for wingless signaling and causes phenotypes in the midgut, notum, and wing that mimic wingless hyperstimulation. Finally, efficient transcriptional stimulation by LEF-1 in the midgut depends also on the decapentaplegic response sequence and is limited spatially by decapentaplegic signaling. Thus, LEF-1 coordinates inputs from multiple positional signals, consistent with its architectural role in regulating the assembly of multiprotein enhancer complexes.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Alleles (15)
	arm² arm^Scer\UAS.cSa dpp^s4 dpp^Scer\UAS.cSa Ecol\lacZ^Ubx.L-CRE Ecol\lacZ^Ubx.ΔBhz.B4 Ecol\lacZ^Ubx.ΔBhz.BG Ecol\lacZ^Ubx.ΔBhz.BL Ecol\lacZ^{Ubx.ΔBhz.L-4} Ecol\lacZ^Ubx.ΔBhz Mmus\Lef1^Scer\UAS.cRa Scer\GAL4^Bx-MS1096 Scer\GAL4^how-24B Scer\GAL4^hs.PB wg^l-17
Constructs (10)
	P{GAL4-Hsp70.PB} P{L-4} P{L-CRE} P{UAS.LEF-1} P{UAS-arm.S} P{UAS-dpp.S} P{Ubx.B4} P{Ubx.BG} P{Ubx.BL} P{Ubx(ΔBhz)lacZ}
Genes (9)
	arm bcd dpp Ecol\lacZ Mmus\Lef1 osk Scer\GAL4 Ubx wg
Insertions (2)
	P{GawB}Bx^MS1096 P{GawB}how^24B