Personal communication from: John Merriam To: Bloomington Drosophila Stock Center Dated: 09 May 1997 Background: Molecular and genetic information on a P construct carried by a stock donated to the Bloomington collection (described on a poster at the 1997 Drosophila Conference). Information communicated: Insert \#4, on the X chromosome, map position about 37. The insert is marked with y+. This insert has the Gal4-UAS sequence and promoter oriented to transcribe leftward out the 5' P end. Further description of the insert: Construct P 6.11 features a promoter and UAS-Gal4 oriented to transcribe leftward from the 5' P end. Inserts in flies are marked with genomic y+ expression. The restriction site map is 5'P BamHI/BglII EcoRI hsp-UAS BamHI SalI (5' y+ genomic) HindIII BglII PstI HindIII BamHI EcoRI PstI BglII BamHI (3' y+ genomic) SalI PstI HindIII 3'P The distance of hsp from 5'P is approximately 140 bp. The hsp-UAS insert from Brand and Perrimon's pUAST is about 400 bp. The genomic y+ insert, from Geyer and Corces, is 8 kb in length (between SalI sites) with transcription from left to right. 3'P is 223 bp from the right SalI site.