Reference Report

Reference
Citation	Hay, B.A., Maile, R., Rubin, G.M. (1997). P element insertion-dependent gene activation in the Drosophila eye. Proc. Natl. Acad. Sci. U.S.A. 94(10): 5195--5200. (Export to RIS)
FlyBase ID	FBrf0093501
Publication Type	Research paper
PubMed ID	9144214
PubMed Abstract	Insights into the function of a gene can be gained in multiple ways, including loss-of-function phenotype, sequence similarity, expression pattern, and by the consequences of its misexpression. Analysis of the phenotypes produced by expression of a gene at an abnormal time, place, or level may provide clues to a gene's function when other approaches are not illuminating. Here we report that an eye-specific, enhancer-promoter present in the P element expression vector pGMR is able to drive high level expression in the eye of genes near the site of P element insertion. Cell fate determination, differentiation, proliferation, and death are essential for normal eye development. Thus the ability to carry out eye-specific misexpression of a significant fraction of genes in the genome, given the dispensability of the eye for viability and fertility of the adult, should provide a powerful approach for identifying regulators of these processes. To test this idea we carried out two overexpression screens for genes that function to regulate cell death. We screened for insertion-dependent dominant phenotypes in a wild-type background, and for dominant modifiers of a reaper overexpression-induced small eye phenotype. Multiple chromosomal loci were identified, including an insertion 5' to hid, a potent inducer of apoptosis, and insertions 5' to DIAP1, a cell death suppressor. To facilitate the cloning of genes near the P element insertion new misexpression vectors were created. A screen with one of these vectors identified eagle as a suppressor of a rough eye phenotype associated with overexpression of an activated Ras1 gene.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Aberrations (1)
	In(2LR)O
Alleles (15)
	BacA\p35^GMR.PH eg²⁸ eg^GMR.PH eg^unspecified GMR^5x.cHa GMR^E.cHa GMR^EP.cHa Hsap\FAS^{GMR.T:Ivir\haemagglutinin} Ras85D^V12.sev rpr^GMR.PH th²²⁸ th³⁵⁵ th^unspecified W¹¹⁰ w^+mC
Balancers (1)
	CyO-CR2
Constructs (11)
	P{GMR} P{GMR-eagle} P{GMR-HAFAS} P{GMRP35} P{GMR-rpr.H} P{Mae-GMR.E} P{Mae-GMR.EP} P{sevRas1.V12} pP{GMR} pP{Mae-GMR.E} pP{Mae-GMR.EP}
Genes (11)
	BacA\p35 eg gl GMR Hsap\FAS Ras85D rpr T:Ivir\haemagglutinin th w W
Insertions (5)
	P{GMR}th²²⁸ P{GMR}th³⁵⁵ P{GMR-HAFAS}W¹¹⁰ P{Mae-GMR.E}eg²⁸ P{sevRas1.V12}FK1