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Citation
Plautz, J.D., Straume, M., Stanewsky, R., Jamison, C.F., Brandes, C., Dowse, H.B., Hall, J.C., Kay, S.A. (1997). Quantitative analysis of Drosophila period gene transcription in living animals.  J. Biol. Rhythms 12(3): 204--217.
FlyBase ID
FBrf0093657
Publication Type
Research paper
Abstract

To determine the in vivo regulatory pattern of the clock gene period (per), the authors recently developed transgenic Drosophila carrying a luciferase cDNA fused to the promoter region of per. They have now carried out noninvasive, high time-resolution experiments allowing high-throughput monitoring of circadian bioluminescence rhythms in individual living adults for several days. This immediately solved several problems (resulting directly from individual asynchrony within a population) that have accompanied previous biochemical experiments in which groups of animals were sacrificed at each time point. Furthermore, the authors have developed numerical analysis methods for automatically determining rhythmicity associated with bioluminescence records from single flies. This has revealed some features of per gene transcription that were previously unappreciated and provides a general strategy for the analysis of rhythmic time series in the study of molecular rhythms.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Biol. Rhythms
    Title
    Journal of Biological Rhythms
    Publication Year
    1986-
    ISBN/ISSN
    0748-7304
    Data From Reference
    Alleles (6)
    Genes (3)
    Experimental Tools (1)
    Transgenic Constructs (3)