Reference Report

Reference
Citation	Strutt, D.I., Weber, U., Mlodzik, M. (1997). The role of RhoA in tissue polarity and Frizzled signalling. Nature 387(6630): 292--295.
FlyBase ID	FBrf0093742
Type of publication	Research paper
Offprint Available	No
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PubMed ID	9153394
PubMed Abstract	The tissue polarity genes of Drosophila are required for correct establishment of planar polarity in epidermal structures, which in the eye is shown in the mirror-image symmetric arrangement of ommatidia relative to the dorsoventral midline. Mutations in the genes frizzled (fz), dishevelled (dsh) and prickle-spiny-legs (pk-sple) result in the loss of this mirror-image symmetry. fz encodes a serpentine receptor-like transmembrane protein required for reception and transmission of a polarity signal. Little else is known of the signalling pathway(s) involved other than that Dsh acts downstream of Fz. We have identified mutations in the Drosophila homologue of RhoA p21 GTPase, and by analysis of their phenotype show that RhoA is required for the generation of tissue polarity. Genetic interactions indicate a role for RhoA in signalling mediated by Fz and Dsh, and furthermore suggest that JNK/SAPK-like kinases are involved. These data are consistent with a Fz/RhoA signalling cascade analogous to the yeast pheromone signalling pathway and that proposed for activation of the serum response factor (SRF) in vertebrate cells.
Biosis	99565202
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Secondary IDs	FBrf0096384
Language of publication	English
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Abbreviation	Nature
Title	Nature
Authors
Volume range	1-
Year range	1869-
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Place of publication	London
Language of publication	English
ISBN/ISSN	0028-0836
CODEN	NATUAS
Data from Reference
Alleles (22)
	Ecol\lacZ^svp-3 Rho1^72BH Rho1^72F Rho1^72M1 Rho1^72O Rho1^72R Rho1^N19.Scer\UAS Rho1^k07236 Rho1^k07903 Scer\GAL4^dpp.blk1 Scer\GAL4^hs.2sev bsk¹ bsk² dsh³ dsh^Scer\UAS.cNa fz¹ fz^Scer\UAS.cAa nmo^E33 pk^pk-sple-13 rl¹⁰ w^+mC wg^l-17
Constructs (5)
	P{GAL4-Hsp70.sev} P{GAL4-dpp.blk1} P{UAS-Rho1.N19} P{UAS-dsh.N} P{UAS-fz.A}
Genes (12)
	Ecol\lacZ Rho1 Scer\GAL4 bsk dsh elav fz nmo pk rl w wg
Insertions (3)
	P{HZ}svp³ P{lacW}Rho1^k07236 P{lacW}Rho1^k07903