Reference Report

Reference
Citation	Hukriede, N.A., Gu, Y., Fleming, R.J. (1997). A dominant-negative form of Serrate acts as a general antagonist of Notch activation. Development 124(17): 3427--3437. (Export to RIS)
FlyBase ID	FBrf0098268
Publication Type	Research paper
PubMed ID	9310337
PubMed Abstract	Specification of the dorsal-ventral compartment boundary in the developing Drosophila wing disc requires activation of NOTCH from its dorsal ligand SERRATE and its ventral ligand DELTA. Both NOTCH ligands are required in this process and one cannot be substituted for the other. In the wing disc, expression of a dominant-negative, truncated form of SERRATE called BD(G), is capable of inhibiting NOTCH activation in the ventral but not the dorsal compartments. We demonstrate that BD(G) can act as a general antagonist of both SERRATE and DELTA mediated NOTCH interactions, however, BD(G) retains the SERRATE protein domain targeted by FRINGE, hence its antagonistic effects are restricted in the dorsal wing disc. Our findings suggest a model in which ligand binding to NOTCH is a necessary but insufficient step toward NOTCH activation.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (3)
	Df(3R)Dl-BX6 Df(3R)Ser^J94 Dp(3;3)bxd¹¹⁰
Alleles (14)
	Dl^Scer\UAS.cJa Ecol\lacZ^ap-rK568 Ecol\lacZ^NUC.Scer\UAS Ecol\lacZ^vg.int2.1 fng^Scer\UAS.cKa Scer\GAL4^e22c Scer\GAL4^hs.PB Scer\GAL4^ptc-559.1 Scer\GAL4^Ser.PGF Ser^+r83k Ser^Bd-3 Ser^{Bd.Scer\UAS.T:Hsap\MYC} Ser^rev6-1 Ser^Scer\UAS.cGa
Constructs (8)
	P{GAL4-Hsp70.PB} P{Ser-GAL4.GF} P{UAS-Bd.H} P{UAS-Dl.J} P{UAS-fng.K} P{UAS-lacZ.NUC} P{UAS-Ser.G} P{vg(D/V)-lacZ}
Genes (7)
	Dl Ecol\lacZ fng N Scer\GAL4 Ser T:Hsap\MYC
Insertions (6)
	P{en2.4-GAL4}e22c P{GawB}ptc^559.1 P{lacZ-un3}Y488 P{PZ}ap^rK568 P{Ser-GAL4.GF}1 P{Ser-GAL4.GF}2