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Reference
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| Citation |
Ohlmeyer, J.T., Kalderon, D. (1997). Dual pathways for induction of wingless expression by protein kinase A and hedgehog in Drosophila embryos. Genes Dev. 11(17): 2250--2258. |
| FlyBase ID |
FBrf0098322 |
| Type of publication |
Research paper |
| Offprint Available |
No |
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External Crossreferences
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| PubMed ID |
9303540 |
| PubMed Abstract |
The secreted Drosophila Hedgehog (Hh) protein induces transcription of specific genes by an unknown mechanism that requires
the serpentine transmembrane protein Smoothened (Smo) and the transcription factor Cubitus interruptus (Ci). Protein kinase
A (PKA) has been implicated in the mechanism of Hh signal transduction because it acts to repress Hh target genes in imaginal
disc cells that express Ci. Changes in Ci protein levels, detected by an antibody that recognizes an epitope in the carboxy-terminal
half of Ci, have been suggested to mediate the positive effects of Hh and the negative effects of PKA on Hh target gene expression
in imaginal discs. Here we show that PKA inhibition, like Hh, leads to increased "carboxy-terminal" Ci staining and Hh target
gene expression in embryos. In addition, we find that Hh and Smo can stimulate target gene expression at constant Ci levels
and that increased PKA activity can induce ectopic Hh target gene expression in a manner that requires Smo and Ci activities
but does not involve changes in Ci protein concentration. This suggests a branching pathway of Hh signal transduction downstream
of Smo and that PKA exerts opposite effects on the two branches. Finally we show that Hh signaling in embryos does not depend
on cAMP-dependent regulation of PKA activity.
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| Biosis |
99752799 |
| Zoological record |
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Associated Information
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| Comments |
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| Text of personal communication |
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Related Publications
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
Genes Dev. |
| Title |
Genes & Development |
| Authors |
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| Volume range |
1- |
| Year range |
1987- |
| Page range |
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| Publisher |
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| Place of publication |
Cold Spring Harbor, NY |
| Language of publication |
English |
| ISBN/ISSN |
0890-9369 |
| CODEN |
GEDEEP |
Data from Reference
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Aberrations (2)
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Alleles (13)
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Constructs (6)
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Genes (11)
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Insertions (3)
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