Reference Report

Reference
Citation	Brand, A. (1997.6.30). P{GawB} insertions. (Export to RIS)
FlyBase ID	FBrf0098595
Publication Type	Personal communication to FlyBase
PubMed ID
PubMed Abstract
Text of Personal Communication	lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000021 GawB: Yes insertion_symbol: P{GawB}h[1J3] chromosome: 3 within_gene: hairy (h) phenotype: recessive; extra scutellar bristles visualization: via P{UAS-lacZ.B} target construct expression: -hairy expression pattern (Brand and Perrimon [1993] Development 118, 401-415. date_updated: Jun 27 1997 3:13:41:623PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000023 GawB: Yes insertion_symbol: P{GawB}24B chromosome: 3 visualization: via P{UAS-lacZ.B} target construct. expression: -Embryonic mesoderm from stage 10 (Brand and Perrimon [1993] Development 118, 401-415. -Eye-antennal, haltere, leg and wing imaginal discs. date_updated: Jun 27 1997 3:16:44:106PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000024 GawB: Yes insertion_symbol: P{GawB}69B chromosome: 3 visualization: via P{UAS-lacZ.B} target construct expression: -Embryonic ectoderm (stripes) from stage 11 -Eye-antennal, haltere, leg and wing imaginal discs (Brand and Perrimon [1993]; Development 118:401-415; Wilder and Perrimon [1995]; Development 121:477-488). date_updated: Jun 27 1997 3:09:45:360PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000025 GawB: Yes insertion_symbol: P{GawB}32B chromosome: 3 visualization: via P{UAS-lacZ.B} target construct) expression: Embryonic ectoderm (stripes), stage 11. Eye-antennal, leg and wing imaginal discs (Brand and Perrimon [1993] 118, 401-419). date_updated: Jun 27 1997 3:45:49:483PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000026 GawB: Yes insertion_symbol: P{GawB}71B chromosome: 3 visualization: via P{UAS-lacZ.B} target construct expression: -Embryonic salivary glands. -Part of dorsal and ventral, posterior wing pouch of wing imaginal discs (excluded from area around margin); very limited expression in eye-antennal imaginal discs (Brand and Perimon [1993] Development 118, 401-415. NOTE: Figure 9, C and D [not A and B, as described in legend]). -Expressed in two dorsal spots in the leg imaginal discs. date_updated: Jun 27 1997 3:58:48:156PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000027 GawB: Yes insertion_symbol: P{GawB}30A chromosome: 2 visualization: via P{UAS-lacZ.B} target construct expression: Embryonic salivary glands, muscle attachment sites. Dorsal sector of the leg imaginal disc. Primarily in the wing hinge region of the wing imaginal disc; dorsal and ventral edges of the eye imaginal disc; single, central dot in antennal disc (Brand and Perrimon [1993] Development 118, 401-415. NOTE: Figure 9A and B [not C and D as described in legend]. insert_comments: See also Ingham and Feitz [1995] Current Biology 5:432-440. date_updated: Jun 27 1997 4:25:15:253PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000028 GawB: Yes insertion_symbol: P{GawB}34B visualization: via P{UAS-lacZ.B} target construct. expression: -Embryonic salivary glands. -Posterior midgut during embryogenesis (unconfirmed). -Eye-antennal, haltere, leg and wing imaginal discs (details undocumented). date_updated: Jun 27 1997 4:30:19:963PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000029 GawB: Yes insertion_symbol: P{GawB}55B chromosome: 3 phenotype: May be a supressor of D-raf (J.A. Kiger, personal communication). visualization: via P{UAS-lacZ.B} target construct. expression: Embryonic salivary glands. Follicle cells (patchy expression) at anterior of oocyte at stage 9 (Brand and Perrimon [1994], Genes and Development 8:629-639). date_updated: Jun 27 1997 4:43:10:736PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000030 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e22c chromosome: 2 visualization: via P{UAS-lacZ.B} target construct. expression: 'Ubiquitous' in embryos. 'Ubiquitous' in imaginal discs. Ovaries (no details available). larval brain, third instar (no details available). insert_comments: This line was generated by A. Brand and K. Yoffe. date_updated: Jun 27 1997 5:41:12:383PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000031 GawB: Yes insertion_symbol: P{GawB}T80 visualization: via P{UAS-lacZ.B} target construct. expression: -Said to be expressed 'ubiquitously' in the imaginal discs at third instar. insert_comments: -Line was isolated by J. Urban and G. Technau. -Insertion is thought to be viable, but this is unconfirmed. date_updated: Jun 27 1997 5:44:48:340PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000032 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e16e chromosome: 2 within_gene: not determined (thought to be in/near engrailed) visualization: via P{UAS-lacZ.B} target construct. expression: engrailed expression pattern. insert_comments: This insertion was generated by A. Brand and K. Yoffe date_updated: Jun 27 1997 5:49:32:596PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000034 GawB: Yes insertion_symbol: P{GawB}10B chromosome: 2 visualization: via P{UAS-lacZ.B} target construct. expression: -No embryonic expression. -In the leg imaginal disc there is a very small spot of expression at the very center of the disc. -In the haltere imaginal disc there are 2-3 very small spots of expression along the A/P border, including one at the center of the disc. -In the wing imaginal disc there are numerous small spots of expression on either side of the D/V border. May include some of the regions where SMC's arise, but does not appear to be restricted to the regions where the SMCs arise. -In the eye/imaginal disc there is a very small spot of expression at the posterior end of the antennal portion of the disc. insert_comments: Generated by A. Brand. date_updated: Jun 27 1997 6:06:46:436PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000035 GawB: Yes insertion_symbol: P{GawB}56B chromosome: 2 visualization: via P{UAS-lacZ.B} target construct. expression: -Embryonic salivary glands and oenocytes. -In the leg imaginal disc there is broad expression throughout, with the possible exception of distal, ventral, posterior regions. -in the eye/antennal imaginal disc, there is broad expression nearly throughout. insert_comments: Generated by A. Brand. date_updated: Jun 27 1997 6:04:09:433PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000038 GawB: Yes insertion_symbol: P{GaWB}38B visualization: via P{UAS-lacZ.B}target construct expression: -Embryonic salivary glands, proventriculus. -At third larval instar, there is a single small dot of expression toward the center of the leg disc. -In the wing disc there are three small spots of expression in the dorsal hinge region and two larger spots on either side of the A/P border in the thoracic region insert_comments: Generated by A. Brand. date_updated: Jun 27 1997 6:17:38:256PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000039 GawB: Yes insertion_symbol: P{GawB}36B chromosome: 3 visualization: via P{UAS-lacZ.B}target construct expression: -Embryonic mesoderm form stage 10. -During third instar there is expression in the leg discs (no details available). -In the wing disc, there is intense expression in the thoracic region, but little or no expression in the wing hinge or wing pouch. insert_comments: Generated by A. Brand. date_updated: Jun 27 1997 6:24:52:276PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000040 GawB: Yes insertion_symbol: P{GawB}33A visualization: via P{UAS-lacZ.B}target construct expression: -Embryonic salivary glands. -At third instar, there is little or no expression in the eye/antennal, wing, leg, and haltere discs -There is expression in the 3rd instar larval brain/ventral ganglion (no further details available) insert_comments: Generated by A. Brand. date_updated: Jun 27 1997 7:06:08:186PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000042 date_updated: Jun 30 1997 12:51:14:316PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000043 GawB: Yes insertion_symbol: P{GawB}6B chromosome: 2 visualization: via P{UAS-lacZ.B}target construct expression: -Embryonic salivary glands. -During third instar, there is expression in the wing (dorsal wing pouch), leg, and eye/antennal discs. In the eye portion of the disc there is expression at the dorsal and ventral edges. In the antennal portion of the disc, about half the disc shows expression (further details not available) insert_comments: Generated by A. Brand date_updated: Jun 30 1997 1:00:13:853PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000045 GawB: Yes insertion_symbol: P{GawB}30B chromosome: 3 visualization: via P{UAS-lacZ.B}target construct expression: -Embryonic salivary glands, pharynx. -At third instar, there is expression throughout the wing pouch and thoracic regions of the disc, except in limited portions of the wing hinge region. There is broad expression in the antennal part of the eye disc and in parts of the eye disc itself. In the leg discs, only the proximal regions do not show expression. insert_comments: Generated by A. Brand. date_updated: Jun 30 1997 3:26:50:566PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000046 GawB: Yes insertion_symbol: P{GawB}54B visualization: via P{UAS-lacZ.B}target construct expression: -Embryonic salivary glands, oenocytes. -At third instar, there is restricted expression in the leg, wing, haltere and eye/antennal imaginal discs (no details available). insert_comments: Generated by A. Brand. date_updated: Jun 30 1997 3:30:12:943PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000047 GawB: Yes insertion_symbol: P{GawB}13M visualization: via P{UAS-lacZ.B}target construct expression: -Embryonic epidermis. -At third instar, there is expression in the leg and eye/antennal imaginal discs (no other details available). insert_comments: Generated by A.Brand. date_updated: Jun 30 1997 3:45:43:106PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000048 chromosome: date_updated: Jun 30 1997 3:48:00:243PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000049 chromosome: date_updated: Jun 30 1997 4:24:09:543PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000052 GawB: Yes insertion_symbol: P{GawB}78B visualization: via P{UAS-lacZ.B} target construct expression: -Embryonic salivary glands. -During third instar, there is no expression in the imaginal discs. -Ovaries. insert_comments: Generated by A. Brand date_updated: Jun 30 1997 4:53:31:873PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000053 GawB: Yes insertion_symbol: P{GawB}28A visualization: via P{UAS-lacZ.B} target construct expression: -Embryonic salivary glands, oenocytes. -At third instar, there is no expression in the imaginal discs. insert_comments: Generated by A.Brand. date_updated: Jun 30 1997 5:05:23:983PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000054 GawB: Yes insertion_symbol: P{GawB}79B visualization: via P{UAS-lacZ.B} target construct expression: -Embryonic salivary glands. -At third instar, there is no expression in the imaginal discs. insert_comments: Generated by A. Brand date_updated: Jun 30 1997 5:09:04:370PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000055 GawB: Yes insertion_symbol: P{GawB}17M visualization: via P{UAS-lacZ.B} target construct expression: -Embryonic salivary glands, yolk. -At third instar, there is no expression in the imaginal discs. -Larval brain (no further details available). insert_comments: Generated by A.Brand. date_updated: Jun 30 1997 5:14:10:210PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000056 GawB: Yes insertion_symbol: P{GawB}46B visualization: via P{UAS-lacZ.B} target construct expression: -Embryonic salivary glands. -At third instar, there is no expression in the imaginal discs. insert_comments: Generated by A.Brand. date_updated: Jun 30 1997 5:22:28:983PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000057 GawB: Yes insertion_symbol: P{GawB}53B visualization: via P{UAS-lacZ.B} target construct expression: -Embryonic salivary glands, antenno-maxillary complex? -During third instar, no expression in imaginal discs. -Ovary. insert_comments: Generated by A.Brand. date_updated: Jun 30 1997 5:30:57:180PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000058 GawB: Yes insertion_symbol: P{GawB}40B visualization: via P{UAS-lacZ.B} target construct expression: -Embryonic salivary glands, muscle attachment sites?. -At third instar, there is no expression in the imaginal discs. insert_comments: Generated by A.Brand. date_updated: Jun 30 1997 5:34:12:810PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000059 GawB: Yes insertion_symbol: P{GawB}58B visualization: via P{UAS-lacZ.B} target construct expression: -Embryonic salivary glands. -At third instar, no expression in the imaginal discs. -Ovary. insert_comments: Generated by A.Brand. date_updated: Jun 30 1997 5:37:50:403PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000060 date_updated: Jun 30 1997 5:39:47:996PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000061 GawB: Yes insertion_symbol: P{GawB}12B visualization: via P{UAS-lacZ.B} target construct expression: -Embryonic salivary glands, mesoderm. -At third instar, no expression in imaginal discs. insert_comments: Generated by A.Brand. date_updated: Jun 30 1997 5:44:42:226PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000063 GawB: Yes insertion_symbol: P{GawB}11A visualization: via P{UAS-lacZ.B} target construct expression: -Embryonic salivary glands, dorsal median cells, dorsal cells at closure. -At third instar, no expression in imaginal discs. insert_comments: Generated by A.Brand. date_updated: Jun 30 1997 6:31:08:040PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000064 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e33C chromosome: 3 visualization: via P{UAS-lacZ.B}target construct expression: During embryogenesis there is expression along the midline of the central nervous system (no further details available.) At third instar there is uniform expression in the lymph glands, as well as expression in the trachea, Malpighian tubules gut, optic lobes and brain (no further details available). insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 6:40:28:006PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000065 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e45A chromosome: 3 visualization: via P{UAS-lacZ.B}target construct expression: -During embryogenesis there are epidermal/mesodermal stripes. (No further details available). -At third instar, there is expression in the eye/antennal and leg discs, and in the brain (no further details available). insert_comments: Generated by A. Brand and K. Yoffe. date_updated: Jun 30 1997 6:39:34:986PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000066 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e43A chromosome: 2 within_gene: engrailed (en) polytene: 48A visualization: via P{UAS-lacZ.B}target construct expression: -During embryogenesis this insertion is expressed in the 'late' engrailed expression pattern (i.e. there is no expression early in embryogenesis) -Expression during larval/pupal stages has not yet been described. insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 6:42:31:206PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000067 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e74A chromosome: 2 within_gene: engrailed (en) polytene: 48A visualization: via P{UAS-lacZ.B}target construct expression: -During embryogenesis this insertion is expressed in the 'late' engrailed expression pattern (i.e. there is no expression at early stage). -At third instar, expression has not yet been determined. insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 6:44:49:006PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000068 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e76B chromosome: 2 visualization: P{UAS-lacZ.B}target construct expression: -During embryogenesis there is expression in epidermal stripes and in the gut (no further details are available). -At third instar, there is little or no expression in the imaginal discs. insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 6:46:15:406PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000069 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}eIT chromosome: 3 visualization: via P{UAS-lacZ.B}target construct expression: -During embryogenesis there is expression in the mesoderm and in the central nervous sytem (no further details available). -Expression during third instar has yet to be determined. insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 6:47:56:606PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000070 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e6A visualization: P{UAS-lacZ.B}target construct expression: -During embryogenesis there is expression in the central nervous system (no further details available). insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 6:49:33:626PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000071 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e1A chromosome: 2 visualization: via P{UAS-lacZ.B}target construct expression: -There is expression during embryogenesis (no further details available). -At third instar, there is 'ubiquitous' expression in the imaginal discs, and expression in the brain (no further details available). insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 6:52:08:626PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000072 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e16A chromosome: 2 visualization: via P{UAS-lacZ.B}target construct expression: During embryogenesis there is expression in the central nervous system (no further details available). -At third instar there is little or no expression in the imaginal discs, but there is expression in the brain (no further details available). insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 6:54:04:226PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000073 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e29C chromosome: 3 visualization: via P{UAS-lacZ.B}target construct expression: -During embryogenesis there is expression in the central nervous system and in the head (no further details are available). -At third instar this insertion is 'ubiquitiously' expressed in the imaginal discs and is also expressed in the brain (no further details available.) insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 6:56:07:226PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000074 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e15A chromosome: 2 within_gene: engrailed (en) polytene: 48A visualization: via P{UAS-lacZ.B)target construct expression: -During embryogenesis this insertion is expressed in the 'late' engrailed expression pattern (i.e. there is no expression early in embryogenesis). -At third instar there is expression in the eye/antennal, haltere, wing and leg imaginal discs (no details available), and in the larval brain (no details available). insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 6:57:34:626PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000075 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e21A chromosome: 2 within_gene: engrailed (en) polytene: 48A visualization: via P{UAS-lacZ.B}target construct expression: -During embryogenesis this insertion is expressed in the 'late' engrailed expression pattern (i.e. there is no expression early in embryogenesis). -At third instar this insertion is expressed in the endogenous engrailed pattern in the imaginal discs, and is expressed in the brain (no details available). insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 7:00:47:646PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000076 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e33A chromosome: 2 within_gene: engrailed (en) polytene: 48A visualization: via P{UAS-lacZ.B}target construct expression: -During embryogenesis this insertion is expressed in the 'late' engrailed expression pattern (ie there is no expression early in embryogenesis). -At third instar there are 'spots' of expression in the posterior compartment of the imaginal discs. There is also expression in the larval brain (no details available). insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 7:06:01:046PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000077 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e8A visualization: via P{UAS-lacZ.B}target construct expression: -During embryogenesis there is expression in the central nervous system (no further details available) -At third instar there is expression in the cuticle, gut and brain (no further details available) insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 7:07:43:066PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000078 GawB: No transposon: P{en-GAL4} transposon_construct: Enhancer trap vector, generated by A. Brand. engrailed upstream sequence ligated to GAL4 coding sequence. insertion_symbol: P{en-GAL4}e13C chromosome: 3 visualization: via P{UAS-lacZ.B}target construct expression: -There is expression during embryogenesis (no further details available). At third instar, there are 'spots' of expression in the posterior compartment of the imaginal discs, as well as in the salivary glands fat bodies, Malpighian tubules and gut (no further details available) insert_comments: Generated by A.Brand and K.Yoffe. date_updated: Jun 30 1997 7:09:23:666PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000079 GawB: Yes insertion_symbol: P{GawB}L chromosome: 1 expression: Embryonic salivary glands. insert_comments: This insertion was used as the 'starter' line for generating novel insertions. Generated by A.Brand. date_updated: Jun 30 1997 7:12:21:043PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000080 GawB: Yes insertion_symbol: P{GawB}19A visualization: via P{UAS-lacZ.B}target construct expression: -Embryonic salivary glands, epidermal stripes. -Third instar, expression anterior to the morphogenetic furrow in eye/antennal disc, in central ring of the antennal disc. In leg disc, expression in a central ring, a large dorsal patch, small dots scattered throughout an external ring (proximal leg fates). In wing discs, four patches of expression along the A/P border, at least 4 patches elsewhere in the wing region, and four patches in the thoracic region of the disc. insert_comments: Generated by A.Brand. date_updated: Jun 30 1997 7:19:58:886PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000081 date_updated: Jun 30 1997 7:21:34:443PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000082 GawB: Yes insertion_symbol: P{GawB}76B visualization: via P{UAS-lacZ.B}target construct expression: -Embryonic salivary glands, ring gland?. -At third instar there is strong pattern expression in the lymph glands as well as expression in the salivary glands, trachea, optic lobes, brain, and imaginal discs (no further details available). insert_comments: Generated by A.Brand. date_updated: Jun 30 1997 7:28:50:703PM -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000094 transposon: P{UAS-Dras2 Val14} vector: pP{UAST} genomic: Ras2, Val14 vector_comments: Generated by A.Brand, described in Brand and Perrimon (1993) Development 118, 401-415. date_updated: Jun 30 1997 7:49:43:760PM ===== insertion_symbol: P{UAS-Dras Val14}B chromosome: 2 insertion_property: viable -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000095 transposon: P{UAS-cRaf1.gof} vector: pP{UAST} other_regulatory: human cRaf1, deletion aa 2-334 (constitutively active) vector_comments: Generated by A. Brand, described in Brand and Perrimon (1994) Genes and Development 8, 629-639. date_updated: Jun 30 1997 7:57:39:100PM ===== insertion_symbol: P{UAS-cRaf1.gof}X chromosome: 1 insertion_property: viable -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000096 transposon: P{UAS-raf.gof} vector: pP{UAST} cDNA: raf, deletion aa 2-431 (constitutively active) vector_comments: Generated by X.Lu, described in Brand and Perrimon (1994) Genes and Development 8, 629-639. date_updated: Jun 30 1997 8:04:51:366PM ===== insertion_symbol: P{UAS-raf.gof}F179 chromosome: 3 insertion_property: viable -------------------------------------------------- lastname : Brand givenname : Andrea institution : Wellcome/CRC Institute email : ahb@XXXXXXXXXXXXXXX FBgu ID : FBgu0000099 transposon: P{UAS-lacZ} vector: pP{UAST} other_regulatory: Bacteria, lacZ vector_comments: Generated by A. Brand, described in Brand and Perrimon (1993) Development 118, 401-415. date_updated: Jun 30 1997 8:09:20:446PM ===== insertion_symbol: P{UAS-lacZ}Bg4-1-2 chromosome: 2 insertion_property: viable ===== insertion_symbol: P{UAS-lacZ}Bg4-2-4b chromosome: 3 insertion_property: viable
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Secondary IDs	FBrf0093871
Language of Publication	English
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Alleles (59)
	Ecol\lacZ^Scer\UAS.cBa en^e15A en^e21A en^e33A en^e43A en^e74A en^e76B h^1J3 Hsap\RAF1^gof.Scer\UAS phl^{Scer\UAS.F179} Ras64B^{Val14.Scer\UAS} Scer\GAL4^6B Scer\GAL4^10B Scer\GAL4^11A Scer\GAL4^12B Scer\GAL4^13M Scer\GAL4^17M Scer\GAL4^19A Scer\GAL4^28A Scer\GAL4^30A Scer\GAL4^30B Scer\GAL4^32B Scer\GAL4^33A Scer\GAL4^34B Scer\GAL4^36B Scer\GAL4^38B Scer\GAL4^40B Scer\GAL4^46B Scer\GAL4^53B Scer\GAL4^54B Scer\GAL4^55B Scer\GAL4^56B Scer\GAL4^58B Scer\GAL4^69B Scer\GAL4^71B Scer\GAL4^76B Scer\GAL4^78B Scer\GAL4^79B Scer\GAL4^e1A Scer\GAL4^e6A Scer\GAL4^e8A Scer\GAL4^e13C Scer\GAL4^e16A Scer\GAL4^e22c Scer\GAL4^e29C Scer\GAL4^e33C Scer\GAL4^e45A Scer\GAL4^eIT Scer\GAL4^en-e15A Scer\GAL4^en-e16E Scer\GAL4^en-e21A Scer\GAL4^en-e33A Scer\GAL4^en-e43A Scer\GAL4^en-e74A Scer\GAL4^en-e76B Scer\GAL4^h-1J3 Scer\GAL4^how-24B Scer\GAL4^l(1)BP-1 Scer\GAL4^T80
Constructs (8)
	P{en2.4-GAL4} P{UAS-cRaf1.gof} P{UAS-lacZ.B} P{UAS-phl.gof} P{UAS-Ras64B.V14} pP{en2.4-GAL4} pP{UAS-cRaf1.gof} pP{UAS-phl.gof}
Genes (7)
	Ecol\lacZ en h Hsap\RAF1 phl Ras64B Scer\GAL4
Insertions (54)
	P{en2.4-GAL4}e1A P{en2.4-GAL4}e6A P{en2.4-GAL4}e8A P{en2.4-GAL4}e13C P{en2.4-GAL4}e16A P{en2.4-GAL4}e16E P{en2.4-GAL4}e22c P{en2.4-GAL4}e29C P{en2.4-GAL4}e33C P{en2.4-GAL4}e45A P{en2.4-GAL4}e76B P{en2.4-GAL4}eIT P{en2.4-GAL4}en^e15A P{en2.4-GAL4}en^e21A P{en2.4-GAL4}en^e33A P{en2.4-GAL4}en^e43A P{en2.4-GAL4}en^e74A P{en2.4-GAL4}en^e76B P{GawB}6B P{GawB}10B P{GawB}11A P{GawB}12B P{GawB}13M P{GawB}17M P{GawB}19A P{GawB}19B P{GawB}28A P{GawB}30A P{GawB}30B P{GawB}32B P{GawB}33A P{GawB}34B P{GawB}36B P{GawB}38B P{GawB}40B P{GawB}46B P{GawB}53B P{GawB}54B P{GawB}56B P{GawB}58B P{GawB}64B P{GawB}69B P{GawB}71B P{GawB}76B P{GawB}78B P{GawB}79B P{GawB}Eip63F-1^55B P{GawB}h^1J3 P{GawB}how^24B P{GawB}l(1)BP¹ P{GawB}T80 P{UAS-cRaf1.gof}X P{UAS-lacZ.B}Bg4-2-4b P{UAS-lacZ.B}melt^Bg4-1-2
Transcripts (8)
	Scer\GAL4^19ARA Scer\GAL4^e45ARA Scer\GAL4^en-e15ARA Scer\GAL4^en-e21ARA Scer\GAL4^en-e33ARA Scer\GAL4^en-e43ARA Scer\GAL4^en-e74ARA Scer\GAL4^en-e76BRA