Reference Report

Reference
Citation	Braun, A., Lemaitre, B., Lanot, R., Zachary, D., Meister, M. (1997). Drosophila immunity: analysis of larval hemocytes by P-element-mediated enhancer trap. Genetics 147(2): 623--634. (Export to RIS)
FlyBase ID	FBrf0098749
Publication Type	Research paper
PubMed ID	9335599
PubMed Abstract	Our aim was to identify new genes involved in the cellular aspects of defense mechanism of Drosophila, as well as in melanotic tumor formation processes that are linked to blood cell disregulation. We have screened 1341 enhancer detector fly lines for expression of the lacZ reporter gene in larval hemocytes at the end of the third instar. We have selected 21 lines in which we observed a reproducible lacZ expression in blood cells. These lines were classified according to the subsets of hemocytes in which lacZ was expressed, and we identified five lines that can be used as lamellocyte markers. Three lines were selected for further analysis. The first exhibited strong lacZ expression in all lamellocytes. The second expressed lacZ in plasmatocytes and lamellocytes, and exhibited a melanotic tumor phenotype in larvae homozygous for the insertion. A third line showed a striking insertion-linked phenotype of melanized lymph glands (the hematopoietic organ), which resulted in the total absence of circulating hemocytes in the mutant larvae. We anticipate that this mutation, which we named domino, will prove a useful tool in the analysis of the role of hemocytes during the various aspects of immune response and melanotic tumor formation.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (3)
	Df(2R)AA21 Df(2R)Pu-D17 In(2LR)O
Alleles (47)
	Bc¹ chic^k02707 CtBP⁰³⁴⁶³ cuc¹ dom^k08108 Ecol\lacZ⁰⁵²⁰³ Ecol\lacZ^chic-k02707 Ecol\lacZ^CtBP-03463 Ecol\lacZ^cuc-1 Ecol\lacZ^{Hsc70-4-03550} Ecol\lacZ^Hsp83-j5C2 Ecol\lacZ^k11328 Ecol\lacZ^klu-10052 Ecol\lacZ^lola-00642 Ecol\lacZ^msn-03349 Ecol\lacZ^msn-06946 Ecol\lacZ^Pka-C1-01272 Ecol\lacZ^Rab11-j2D1 Ecol\lacZ^shk-1 Ecol\lacZ^spin-10403 Ecol\lacZ^stg-01235 Ecol\lacZ^Ten-m-05309 Ecol\lacZ^th-j5C8 Ecol\lacZ^tws-02414 Ecol\lacZ^zfh1-00865 esg⁰⁵⁷²⁹ hop^Tum Hsc70-4⁰³⁵⁵⁰ Hsp83^j5C2 klu¹⁰⁰⁵² l(2)k11328^k11328 lola⁰⁰⁶⁴² Mmus\Nfkb1^{RHR.Hsp83.T:Ecol\lacZ} msn⁰³³⁴⁹ msn⁰⁶⁹⁴⁶ Pka-C1⁰¹²⁷² Rab11^j2D1 shk¹ spen⁰³³⁵⁰ spin¹⁰⁴⁰³ sra⁰⁵²⁰³ stg⁰¹²³⁵ Ten-m⁰⁵³⁰⁹ th^j5C8 Tl⁸ tws⁰²⁴¹⁴ zfh1⁰⁰⁸⁶⁵
Balancers (1)
	CyO-vKa
Constructs (1)
	P{p50-lacZ}
Genes (29)
	Bc chic crq CtBP cuc dom Ecol\lacZ esg hop Hsc70-4 Hsp83 klu l(2)k11328 lola Mmus\Nfkb1 msn Pka-C1 Rab11 shk spen spin sra stg T:Ecol\lacZ Ten-m th Tl tws zfh1
Insertions (23)
	P{lacW}chic^k02707 P{lacW}dom^k08108 P{lacW}Hsp83^j5C2 P{lacW}l(2)k11328^k11328 P{lacW}Rab11^j2D1 P{lacW}th^j5C8 P{PZ}CtBP⁰³⁴⁶³ P{PZ}cuc¹ P{PZ}esg⁰⁵⁷²⁹ P{PZ}Hsc70-4⁰³⁵⁵⁰ P{PZ}klu¹⁰⁰⁵² P{PZ}lola⁰⁰⁶⁴² P{PZ}msn⁰³³⁴⁹ P{PZ}msn⁰⁶⁹⁴⁶ P{PZ}Pka-C1⁰¹²⁷² P{PZ}shk¹ P{PZ}spen⁰³³⁵⁰ P{PZ}spin¹⁰⁴⁰³ P{PZ}sra⁰⁵²⁰³ P{PZ}stg⁰¹²³⁵ P{PZ}Ten-m⁰⁵³⁰⁹ P{PZ}tws⁰²⁴¹⁴ P{PZ}zfh1⁰⁰⁸⁶⁵