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Shigenaga, A., Kimura, K., Kobayakawa, Y., Tsujimoto, Y., Tanimura, T. (1997). Cell ablation by ectopic expression of cell death genes, ced-3 and Ice, in Drosophila.  Dev. Growth Differ. 39(4): 429--436.
FlyBase ID
FBrf0098884
Publication Type
Research paper
Abstract

We have developed a system for killing specific cells in Drosophila using ectopic expression of cell death genes. CED-3 and ICE (caspase-1) are proteins required for programmed cell death in the nematode Caenorhabditis elegans and in mammals, respectively. Our previous study has shown that both ced-3 and Ice can elicit cell death in Drosophila. By expressing ced-3 or Ice in several kinds of cells using a GAL4-UAS system and examining the resulting morphological defects, we show that these abnormalities are thought to be caused by the action of ced-3 or Ice genes. As cells are killed by apoptosis in our system, we could eliminate the possibility of harmful effects on the neighboring cells. Our system provides an alternative and novel cell ablation method to elucidate mechanisms of cell differentiation and cell-cell interactions during development in Drosophila.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Growth Differ.
    Title
    Development, Growth and Differentiation
    Publication Year
    1969-
    ISBN/ISSN
    0012-1592
    Data From Reference
    Alleles (7)
    Genes (5)
    Human Disease Models (1)
    Insertions (3)
    Transgenic Constructs (3)