Reference Report

Reference
Citation	Simmonds, A., Hughes, S., Tse, J., Cocquyt, S., Bell, J. (1997). The effect of dominant vestigial alleles upon vestigial-mediated wing patterning during development of Drosophila melanogaster. Mech. Dev. 67(1): 17--33. (Export to RIS)
FlyBase ID	FBrf0098887
Publication Type	Research paper
PubMed ID	9347912
PubMed Abstract	The vestigial gene product is required for the completion of wing development in Drosophila melanogaster. In the absence of vestigial gene expression, cells within the larval wing and haltere imaginal discs fail to proliferate normally thus producing adults with severely reduced wings. Of a large number of vestigial mutations that have been characterized, only two are currently known to exist, vestigial(U) and vestigial(W), which manifest a significant dominant phenotype. Both are associated with chromosomal inversions that fuse the majority of the vestigial coding regions to other genes; mastermind in vestigial(U) and invected in vestigial(W) Examination of vestigial expression in the presence of these dominant alleles shows alterations in the disc-specific expression of vestigial during later stages of larval development. These patterning disruptions are specific to cells of the wing imaginal disc, as significant suppression of total levels of vestigial expression within entire larvae could not be detected. This dominant interference of vestigial patterning appears to be mediated in part by the vestigial coding sequences that are within the gene fusions. Further evidence that the dominant phenotype is the result of disrupted vestigial patterning comes from observations that the dominant alleles can be partially suppressed by mutations within the Drosophila-epidermal growth factor receptor gene. Mutagenesis of vestigial(U) and vestigial(W) produced a series of alleles with partially dominant phenotypes that restored various amounts of the adult wing. These phenotypes can be correlated with alterations in specific portions of the vestigial sequences associated with the dominant alleles. In the presence of these partially dominant alleles, wing imaginal discs have significantly more cells which express vestigial compared with the number associated with the original dominant phenotype. Additionally, eliminating some of the dominant effect causes alterations in the patterns of early stage apoptotic cell death associated with dominant vestigial alleles. Utilizing these new vestigial alleles, it is possible to correlate the consequence of altered vestigial expression to subsequent changes in patterning of the wing disc.
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Mech. Dev.
Title	Mechanisms of Development
Publication Year	1990-
ISBN/ISSN	0925-4773
Data from Reference
Aberrations (24)
	Df(2R)vg-B In(2R)vg^U In(2R)vg^URK1 In(2R)vg^URK2 In(2R)vg^URK3 In(2R)vg^URK4 In(2R)vg^URK5 In(2R)vg^URK6 In(2R)vg^URK7 In(2R)vg^URK9 In(2R)vg^URK11 In(2R)vg^URK12 In(2R)vg^URK13 In(2R)vg^URK14 In(2R)vg^URK15 In(2R)vg^URK16 In(2R)vg^URK17 In(2R)vg^W In(2R)vg^WRK1 In(2R)vg^WRK2 In(2R)vg^WRK3 In(2R)vg^WRK4 In(2R)vg^WRK5 In(2R)vg^WRK6
Alleles (52)
	Ecol\lacZ^ap-rK568 Ecol\lacZ^sd-ETX4 Ecol\lacZ^vg.806 Ecol\lacZ^vg.int2.1 Egfr^unspecified inv^W inv^WRK1 inv^WRK2 inv^WRK3 inv^WRK4 inv^WRK5 inv^WRK6 mam^U mam^URK1 mam^URK2 mam^URK3 mam^URK4 mam^URK5 mam^URK6 mam^URK7 mam^URK9 mam^URK11 mam^URK12 mam^URK13 mam^URK14 mam^URK15 mam^URK16 mam^URK17 vg^83b27-R vg^U vg^URK1 vg^URK2 vg^URK3 vg^URK4 vg^URK5 vg^URK6 vg^URK7 vg^URK9 vg^URK11 vg^URK12 vg^URK13 vg^URK14 vg^URK15 vg^URK16 vg^URK17 vg^W vg^WRK1 vg^WRK2 vg^WRK3 vg^WRK4 vg^WRK5 vg^WRK6
Constructs (2)
	P{vg(806)-lacZ} P{vg(D/V)-lacZ}
Genes (7)
	Ecol\lacZ Egfr inv mam sd vg wg
Insertions (2)
	P{lArB}sd^ETX4 P{PZ}ap^rK568