Open Close
Reference
Citation
Klapper, M., Exner, K., Kempf, A., Gehrig, C., Stuurman, N., Fisher, P., Krohne, G. (1997). Assembly of A- and B-type lamins studied in vivo with the baculovirus system.  J. Cell Sci. 110(20): 2519--2532.
FlyBase ID
FBrf0099049
Publication Type
Research paper
Abstract
We have expressed an A-type lamin (Xenopus lamin A), a probable A-type lamin (Drosophila lamin C), two B-type lamins (Xenopus lamin LI, Drosophila lamin Dmo), and two mutants of Xenopus lamin A in Sf9 cells. All proteins were synthesized at high levels resulting in formation of paracrystals with an axial repeat of 18.5-20.0 nm by A-type lamins; in contrast B-type lamins assembled into aggregates with a fibrillar ultrastructure. Of the four wild-type proteins analyzed only lamin Dmo was found in the nuclear compartment of Sf9 cells in association with the lamina whereas the three other lamins assembled into polymers localized in the cytoplasm as well as the nucleoplasm. The Xenopus lamin A mutant lacking the complete carboxy-terminal tail assembled in the cytoplasm into long filament bundles consisting of fibrils of less than 6 nm diameter. In vitro the non-helical amino-terminal head domain of lamins is required for the formation of 'head-to-tail' polymers. A lamin A mutant lacking this domain could be efficiently extracted from Sf9 cells with physiological buffers containing Triton X-100, demonstrating the importance of this domain for lamin assembly in vivo.
PubMed ID
PubMed Central ID
DOI
Related Publication(s)
Erratum
Assembly of A- and B-type lamins studied in vivo with the baculovirus system.
Klapper et al., 1998, J. Cell Sci. 111(12): 1 after 1766 [FBrf0103431]
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
  • FBrf0099028
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
J. Cell Sci.
Title
Journal of Cell Science
Publication Year
1966-
ISBN/ISSN
0021-9533
Data From Reference
Genes (2)