Reference Report

Reference
Citation	Hammond, L.E., Rudner, D.Z., Kanaar, R., Rio, D.C. (1997). Mutations in the hrp48 gene, which encodes a Drosophila heterogeneous nuclear ribonucleoprotein particle protein, cause lethality and developmental defects and affect P-element third-intron splicing in vivo. Mol. Cell. Biol. 17(12): 7260--7267. (Export to RIS)
FlyBase ID	FBrf0099247
Publication Type	Research paper
PubMed ID	9372958
PubMed Abstract	The Drosophila melanogaster hnRNP protein, hrp48, is an abundant heterogeneous nuclear RNA-associated protein. Previous biochemical studies have implicated hrp48 as a component of a ribonucleoprotein complex involved in the regulation of the tissue-specific alternative splicing of the P-element third intron (IVS3). We have taken a genetic approach to analyzing the role of hrp48. Mutations in the hrp48 gene were identified and characterized. hrp48 is an essential gene. Hypomorphic mutations which reduce the level of hrp48 protein display developmental defects, including reduced numbers of ommatidia in the eye and morphological bristle abnormalities. Using a P-element third-intron reporter transgene, we found that reduced levels of hrp48 partially relieve IVS3 splicing inhibition in somatic cells. This is the first direct evidence that hrp48 plays a functional role in IVS3 splicing inhibition.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Alleles (10)
	Hrb27C⁰²⁶⁴⁷ Hrb27C¹⁰²⁸⁰ Hrb27C^Act5C.PH Hrb27C^hs.PH Hrb27C^k10413 Hrb27C^k16203 Hrb27C^rF680 P\T^{IVS3.hs.T:Ecol\lacZ} w^+mC w^+mW.hs
Constructs (3)
	P{Act5C-Hrb27C.H} P{hs-Hrb27C.H} P{hsp70-IVS3-βgeo}
Genes (4)
	Hrb27C P\T T:Ecol\lacZ w
Insertions (5)
	P{lacW}Hrb27C^k10413 P{lacW}Hrb27C^k16203 P{PZ}Hrb27C⁰²⁶⁴⁷ P{PZ}Hrb27C¹⁰²⁸⁰ P{PZ}Hrb27C^rF680
Natural transposons (1)
	P-element