Reference Report

Reference
Citation	Barrett, K., Leptin, M., Settleman, J. (1997). The Rho GTPase and a putative RhoGEF mediate a signaling pathway for the cell shape changes in Drosophila gastrulation. Cell 91(7): 905--915. (Export to RIS)
FlyBase ID	FBrf0099954
Publication Type	Research paper
PubMed ID	9428514
PubMed Abstract	The Rho GTPases mediate actin rearrangements that are likely to be required for the numerous cell shape changes in a developing embryo. In a genetic screen for Rho signaling pathway components in Drosophila, we identified a gene, DRhoGEF2, that encodes a predicted Rho-specific guanine nucleotide exchange factor. Embryos lacking DRhoGEF2 fail to gastrulate due to a defect in cell shape changes required for tissue invagination, and expression of a dominant-negative Rho GTPase in early embryos results in similar defects. Evidence is also presented that DRhoGEF2 mediates these specific cell shape changes in response to the extracellular ligand, Fog. Together, these results establish a Rho-mediated signaling pathway that is essential for the major morphogenetic events in Drosophila gastrulation.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Alleles (23)
	Cdc42^N17.Scer\UAS Ecol\lacZ^ftz.PC Ecol\lacZ^sim.P3.7 fog^hkb.PB Rac1^N17.Scer\UAS Rho1^GMR.PH Rho1^N19.Scer\UAS RhoGEF2^1.1 RhoGEF2^2.3 RhoGEF2^2.6 RhoGEF2^2.9 RhoGEF2^4.1 RhoGEF2^4.4 RhoGEF2^5.1 RhoGEF2^5.2 RhoGEF2^6.5 RhoGEF2^8.1 RhoGEF2^8.9 RhoGEF2^9.6 RhoGEF2⁰⁴²⁹¹ Scer\GAL4^nos.PG w^+mC w^+mW.hs
Constructs (8)
	P{3.7sim/lacZ} P{ftz/lacC} P{GAL4-nos.NGT} P{GMR-Rho1} P{hkb-fog.B} P{UAS-Cdc42.N17} P{UAS-Rac1.N17} P{UAS-Rho1.N19}
Genes (11)
	c Cdc42 Ecol\lacZ fog px Rac1 Rho1 RhoGEF2 Scer\GAL4 twi w
Insertions (2)
	P{GAL4-nos.NGT}40 P{PZ}RhoGEF2⁰⁴²⁹¹