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Citation
Liu, Z.P., Galindo, R.L., Wasserman, S.A. (1997). A role for CKII phosphorylation of the cactus PEST domain in dorsoventral patterning of the Drosophila embryo.  Genes Dev. 11(24): 3413--3422.
FlyBase ID
FBrf0100135
Publication Type
Research paper
Abstract

Regulated proteolysis of Cactus, the cytoplasmic inhibitor of the Rel-related transcription factor Dorsal, is an essential step in patterning of the Drosophila embryo. Signal-induced Cactus degradation frees Dorsal for nuclear translocation on the ventral and lateral sides of the embryo, establishing zones of gene expression along the dorsoventral axis. Cactus stability is regulated by amino-terminal serine residues necessary for signal responsiveness, as well as by a carboxy-terminal PEST domain. We have identified Drosophila casein kinase II (CKII) as a Cactus kinase and shown that CKII specifically phosphorylates a set of serine residues within the Cactus PEST domain. These serines are phosphorylated in vivo and are required for wild-type Cactus activity. Conversion of these serines to alanine or glutamic acid residues differentially affects the levels and activity of Cactus in embryos, but does not inhibit the binding of Cactus to Dorsal. Taken together, these data indicate that wild-type axis formation requires CKII-catalyzed phosphorylation of the Cactus PEST domain.

PubMed ID
PubMed Central ID
PMC316825 (PMC) (EuropePMC)
Related Publication(s)
Review

Paper Alert.
Anonymous, 1998, Curr. Opin. Genet. Dev. 8(1): 6 [FBrf0100762]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genes Dev.
    Title
    Genes & Development
    Publication Year
    1987-
    ISBN/ISSN
    0890-9369
    Data From Reference
    Alleles (2)
    Genes (6)