Home
Reference Report
| Reference | |||
|---|---|---|---|
| Citation | Semionov, E.P., Markova, B. (1997). Directed alterations of the X-chromosomal ribosomal gene repeats in mutant sc8 of Drosophila melanogaster. Genetica 101(1): 35--40. (Export to RIS) | ||
| FlyBase ID | FBrf0100388 | ||
| Publication Type | Research paper | ||
| PubMed ID | 9465408 | ||
| PubMed Abstract | The patterns of the ribosomal DNA (rDNA) repeat units in seven Drosophila melanogaster inversional mutants have been studied. Among them, only the In(1)sc8 and its deletional derivative Df(1)mal12 female rDNAs exhibited significant reduction in the size of nearly all units, compared to the wild-type females (Canton S, Oregon R). Further investigation shows that each kind of repeat (insertion-free, insertion-containing) in the Xsc8 rDNA array is highly enriched with short (reduced to 4 kilobases) intergenic spacers (IGSs). We revealed two main types of rearrangements. Only part of the 4 kb IGSs display variable length deletions (0.2-0.6 kb) at the 5' ends, within the so-called '1900' base pair (bp) region, recognizable by restriction endonuclease AluI. The presence of additional 100-150 bp DNA in the start portion of this region has also been demonstrated. In contrast, the 3' end spacer regions, corresponding to the external transcribed spacer, do not show any changes in size. These data indicate how reductions of approximately 1.1 kb DNAs in sc8 IGSs, carrying both the rearranged and non-rearranged '1900' sequences, are achieved: the fixed decrease of a number of 240 bp AluI subrepeats, clustered in the central IGS portion, also contribute. None of the other similar inversional mutants examined has so many IGS variants. Therefore, alterations in the Xsc8 rRNA gene cluster seem not to be dependent on its inversional status. | ||
| DOI | |||
| Related Publication(s) | |||
Recent Updates
|
|||
| Description |
What does this section display?
This section contains items that were added to this record for each release.
It currently only tracks new links between this FlyBase report and other
FlyBase data classes (e.g. genes, references, stocks) or controlled
vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
|
||
| Update Feed |
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your
feed reader.
|
||
| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
|
Associated Information
|
|||
| Comments | |||
| Associated Files | |||
|
Other Information
|
|||
| Secondary IDs | |||
| Language of Publication | English | ||
| Additional Languages of Abstract | |||
| Also Published As | |||
|
Parent Publication
|
|||
| Publication Type | Journal | ||
| Abbreviation | Genetica | ||
| Title | Genetica | ||
| Publication Year | 1919- | ||
| ISBN/ISSN | 0016-6707 | ||
|
Data from Reference
|
|||
| Aberrations (6)
|
|||
| Genes (1)
|
|||
| Natural transposons (2)
|
|||