Reference Report

Reference
Citation	Elfring, L.K., Daniel, C., Papoulas, O., Deuring, R., Sarte, M., Moseley, S., Beek, S.J., Waldrip, W.R., Daubresse, G., DePace, A., Kennison, J.A., Tamkun, J.W. (1998). Genetic analysis of brahma: the Drosophila homolog of the yeast chromatin remodeling factor SWI2/SNF2. Genetics 148(1): 251--265. (Export to RIS)
FlyBase ID	FBrf0100560
Publication Type	Research paper
PubMed ID	9475737
PubMed Abstract	The Drosophila brahma (brm) gene encodes an activator of homeotic genes related to the yeast chromatin remodeling factor SWI2/SNF2. Here, we report the phenotype of null and dominant-negative brm mutations. Using mosaic analysis, we found that the complete loss of brm function decreases cell viability and causes defects in the peripheral nervous system of the adult. A dominant-negative brm mutation was generated by replacing a conserved lysine in the ATP-binding site of the BRM protein with an arginine. This mutation eliminates brm function in vivo but does not affect assembly of the 2-MD BRM complex. Expression of the dominant-negative BRM protein caused peripheral nervous system defects, homeotic transformations, and decreased viability. Consistent with these findings, the BRM protein is expressed at relatively high levels in nuclei throughout the developing organism. Site-directed mutagenesis was used to investigate the functions of conserved regions of the BRM protein. Domain II is essential for brm function and is required for the assembly or stability of the BRM complex. In spite of its conservation in numerous eukaryotic regulatory proteins, the deletion of the bromodomain of the BRM protein has no discernible phenotype.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (1)
	Df(3L)th102
Alleles (13)
	brm¹ brm² brm^+t14.4 brm^{K804R.Scer\UAS.T:Ivir\HA1} brm^{K804R.T:Ivir\HA1} brm^T:Ivir\HA1 brm^{Δ549-610.T:Ivir\HA1} brm^Δ1446-1517 Ecol\lacZ^Scer\UAS.cBa Scer\GAL4^69B Scer\GAL4^en-e16E Scer\GAL4^h-1J3 w^+mC
Constructs (7)
	P{9222-3brm-HA} P{BR14.4} P{brm^K804R.T:HA1} P{brm^Δ549-610} P{brm^Δ1446-1517} P{UAS-brm.K804R.T:HA1} P{UAS-lacZ.B}
Genes (5)
	brm Ecol\lacZ Scer\GAL4 T:Ivir\HA1 w
Insertions (3)
	P{en2.4-GAL4}e16E P{GawB}69B P{GawB}h^1J3