Reference Report

Reference
Citation	Li, X., Graner, M.W., Williams, E.L., Roote, C.E., Bunch, T.A., Zusman, S. (1998). Requirements for the cytoplasmic domain of the alphaPS1, alphaPS2 and betaPS integrin subunits during Drosophila development. Development 125(4): 701--711. (Export to RIS)
FlyBase ID	FBrf0101940
Publication Type	Research paper
PubMed ID	9435290
PubMed Abstract	The integrins are a family of transmembrane heterodimeric proteins that mediate adhesive interactions and participate in signaling across the plasma membrane. In this study we examine the functional significance of the cytoplasmic domains of the alphaPS1, alphaPS2 and betaPS subunits of the Drosophila Position Specific (PS) integrin family by analyzing the relationship between cytoplasmic domain structure and function in the context of a developing organism. By examining the ability of ssPS molecules lacking the cytoplasmic domain to rescue embryonic abnormalities associated with PS integrin loss, we find that although many embryonic events require the betaPS cytoplasmic domain, this portion of the molecule is not required for at least two processes requiring PS integrins: formation of midgut constrictions and maintaining germband integrity. Furthermore, our studies demonstrate that mutant proteins affecting four highly conserved amino acid residues in the cytoplasmic tail function with different efficiencies during embryonic development, suggesting that interaction of PS integrins with cytoplasmic ligands is developmentally modulated during embryogenesis. We have also examined the ability of alphaPS1 and alphaPS2 to function without their cytoplasmic domains. By analyzing the ability of transgenes producing truncated alphaPS molecules to rescue abnormalities associated with integrin loss, we find that the cytoplasmic tail of alphaPS2 is essential for both embryonic and postembryonic processes, while this portion of alphaPS1 is not required for function in the wing and in the retina. Furthermore, temperature-shift experiments suggest roles for the alphaPS2 cytoplasmic domain in signaling events occurring in the developing wing.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (25)
	if^C.hs if^C.Scer\UAS if^k27e if^m8.hs if^m8.Scer\UAS if^Δ.C.hs if^{Δ.C.Scer\UAS} if^Δ.m8.hs if^{Δ.m8.Scer\UAS} mew⁰²³ mew⁴⁹⁸ mew^Scer\UAS.cWa mew^Δ.hs mew^Δ.Scer\UAS mys¹¹ mys^hs.PF mys^rDEA mys^rFFA mys^rYYF mys^t1 mys^t2 mys^tZa Scer\GAL4⁶⁸⁴ Scer\GAL4^how-24B Scer\GAL4^hs.PB
Constructs (13)
	P{GAL4-Hsp70.PB} P{mys⁺10.5} P{mys^rDEA} P{mys^rFFA} P{mys^rYYF} P{mys^t1} P{mys^t2} P{UAS-if.C.Δ} P{UAS-if.C} P{UAS-if.m8.Δ} P{UAS-if.m8} P{UAS-mew.W} P{UAS-mew.Δ}
Genes (4)
	if mew mys Scer\GAL4
Insertions (2)
	P{GAL4}684 P{GawB}how^24B