Reference Report

Reference
Citation	Ward, R.E., Lamb, R.S., Fehon, R.G. (1998). A conserved functional domain of Drosophila coracle is required for localization at the septate junction and has membrane-organizing activity. J. Cell Biol. 140(6): 1463--1473. (Export to RIS)
FlyBase ID	FBrf0102020
Publication Type	Research paper
PubMed ID	9508778
PubMed Abstract	The protein 4.1 superfamily is comprised of a diverse group of cytoplasmic proteins, many of which have been shown to associate with the plasma membrane via binding to specific transmembrane proteins. Coracle, a Drosophila protein 4.1 homologue, is required during embryogenesis and is localized to the cytoplasmic face of the septate junction in epithelial cells. Using in vitro mutagenesis, we demonstrate that the amino-terminal 383 amino acids of Coracle define a functional domain that is both necessary and sufficient for proper septate junction localization in transgenic embryos. Genetic mutations within this domain disrupt the subcellular localization of Coracle and severely affect its genetic function, indicating that correct subcellular localization is essential for Coracle function. Furthermore, the localization of Coracle and the transmembrane protein Neurexin to the septate junction display an interdependent relationship, suggesting that Coracle and Neurexin interact with one another at the cytoplasmic face of the septate junction. Consistent with this notion, immunoprecipitation and in vitro binding studies demonstrate that the amino-terminal 383 amino acids of Coracle and cytoplasmic domain of Neurexin interact directly. Together these results indicate that Coracle provides essential membrane-organizing functions at the septate junction, and that these functions are carried out by an amino-terminal domain that is conserved in all protein 4.1 superfamily members.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (1)
	Df(3L)vin7
Alleles (18)
	cora^{1-167.hs.T:Hsap\MYC} cora^{1-323.hs.T:Hsap\MYC} cora^{1-383.hs.T:Hsap\MYC} cora² cora⁴ cora⁵ cora⁶ cora¹⁰ cora^{106-167.hs.T:Hsap\MYC} cora^{106-383.hs.T:Hsap\MYC} cora^{207-383.hs.T:Hsap\MYC} cora^{378-1591.hs.T:Hsap\MYC} cora^{378-1698.hs.T:Hsap\MYC} cora^{1585-1698.hs.T:Hsap\MYC} cora^{fl.hs.T:Hsap\MYC} Nrx-IV⁴⁶ Nrx-IV⁴⁰²⁵ Nrx-IV⁴³⁰⁴
Constructs (10)
	P{hs-cora.1-167.T:Myc} P{hs-cora.1-323.T:Myc} P{hs-cora.1-383.T:Myc} P{hs-cora.106-167.T:Myc} P{hs-cora.106-383.T:Myc} P{hs-cora.207-383.T:Myc} P{hs-cora.378-1591.T:Myc} P{hs-cora.378-1698.T:Myc} P{hs-cora.1585-1698.T:Myc} P{hs-cora.fl.T:Myc}
Genes (4)
	cora dlg1 Nrx-IV T:Hsap\MYC