Subject: ADRC: 254B Dear Syed, I am writing in connection with your abstract for the upcoming Washington DC ADRC: 'Mutations in the obake gene lead to significant head deformities.' You describe a gene, obake, that is new to FlyBase. Do you have a map location for obake? It is nice if we can keep as many gene records as possible anchored to the map. Thank you for your help, with best wishes, Rachel. Subject: ADRC: 255C Dear Janet, I am writing in connection with your abstract for the upcoming Washington DC ADRC: 'hunchback-like (hbl) encodes a ubiquitin conjugating enzyme and potentially functions as a Gap gene.' You describe a gene, hbl, that is new to FlyBase. Do you have a map location for hbl? It is nice if we can keep as many gene records as possible anchored to the map. You also describe an hbl mutant allele in some detail (the PZ insertion). Do you have a symbol for this allele? Thank you for your help, with best wishes, Rachel. Subject: ADRC: 256A Dear Soichi, I am writing in connection with your abstract for the upcoming Washington DC ADRC: 'oroshigane (oro), a segment polarity gene, encodes a multiple pass transmembrane protein with conserved phosphorylation sites for both Protein Kinase A and Casein Kinase II.' You wrote: 'This protein has 52\% identity to the 52.8 KD hypothetical protein isolated from C. elegans, and 33\% identity to the 67 KD hypothetical protein isolated from Yeast.' We would be most grateful if you could tell us the names of the worm and yeast genes. Thank you for your help, with best wishes, Rachel. Subject: ADRC: 254B, 255C, 256A Dear Rachel, I am responsible for three memo that you sent to my lab. 254B, obake mutation. We mapped this mutation in 48B2;48B7 based on our complementation tests using Df(2R)en spx31, Df(2R)enA and Df(2R)enB. Obake is not allelic to en based on our complementation tests using en1 and Df(2R)enE. 255C, hunchback-like. We renamed it to semushi. Its symbol is semi. Location:21C6-7 . This is l(2)02858 in FlyBase. Bloomington stock# is P1214. The deduced semushi protein shows the highest homology with human ubiquitin-conjugated enzyme E2 (acc#P50550). 256A, oroshigane, They do not have names yet. They are the sons/daughters of genome projects. They are listed as hypothetical proteins. The C. elegans one is hypothetical 52.8 KD protein (Acc#; P49049) The yeast one is hypothetical 67.5 KD protein (Acc#;P34248) If you need more, please let me know. Best wishes. Soichi Subject: Re: ADRC: 254B, 255C, 256A Dear Soichi, I will curate your mail as a personal communication to FlyBase, as the source of the map data etc. 254B: obake. Just checking the details -- I think the results will have been that Df(2R)en-A and Df(2R)en-B complement (do not delete) obake, and that Df(2R)en-SFX31 (which I presume corresponds to your 'Df(2R)en spx31') fails to complement (does delete) obake. Please could you confirm this for me? 255C: The 'hbl' allele will have the symbol semi02858 Very best wishes, Rachel. Subject: Re: ADRC: 254B, 255C, 256A Rachel, What you wrote is fine, but I would like to make sure that you change the name of semi02858 from hunchback-like to semushi (semushi means hunchback in Japanese). Thanks, Soichi Subject: Re: ADRC: 254B, 255C, 256A Hi Soichi, >change >the name of semi02858 from hunchback-like to semushi Indeed, semi will be the valid symbol for the gene referred to as 'hbl: hunchback-like' in your abstract. >semushi means >hunchback in Japanese thank you for this little bit extra, I will add it to the data. Rachel.
