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Reference
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| Citation |
Hernandez, G., del Mar Castellano, M., Agudo, M., Sierra, J.M. (1998). Isolation and characterization of the cDNA and the gene for eukaryotic translation initiation factor 4G from Drosophila melanogaster. Europ. J. Biochem. 253(1): 27--35. |
| FlyBase ID |
FBrf0102330 |
| Type of publication |
Research paper |
| Offprint Available |
Yes |
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External Crossreferences
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| PubMed ID |
9578457 |
| PubMed Abstract |
Recent evidence supports the notion that the eukaryotic polypeptide chain initiation factor (eIF)4G plays a critical bridging
role in coordinating other eIF involved in eukaryotic translation initiation. Here we report the isolation and characterization
of a 5621-bp cDNA encoding Drosophila eIF4G. The longest ORF predicts a polypeptide of 1666 amino acids with a molecular mass
of 183,940 Da and shares 25% amino acid identity with other eIF4G. The 5' untranslated region is 386 nucleotides long and
contains seven AUG codons out of frame. The in vitro transcription/translation of the cDNA yielded a major polypeptide, which
was specifically immunoprecipitated with an antibody against Drosophila eIF4G. This polypeptide has the same electrophoretic
mobility as eIF4G purified from Drosophila melanogaster embryos. A conserved eIF4E-binding motif was found in Drosophila eIF4G.
The gene maps at the 102E region of chromosome 4 and spans a genomic region of approximately 16 kb. It was found to contain
15 introns. A single RNA transcript of approximately 5.9 kb was detected by northern blotting of poly(A)-rich RNA prepared
from Drosophila adults. The sequence upstream of the transcription initiation site lacks the consensus TATA box, but contains
several sequences possibly involved in the regulation of transcription.
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| Biosis |
1211457 |
| Zoological record |
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Associated Information
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| Text of personal communication |
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
Europ. J. Biochem. |
| Title |
European Journal of Biochemistry |
| Authors |
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| Volume range |
1-271 |
| Year range |
1967-2004 |
| Page range |
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| Publisher |
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| Place of publication |
Berlin |
| Language of publication |
English |
| ISBN/ISSN |
0014-2956 |
| CODEN |
EJBCAI |
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Data from Reference
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| Genes (2)
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