Reference Report

Reference
Citation	Duffy, J.B., Harrison, D.A., Perrimon, N. (1998). Identifying loci required for follicular patterning using directed mosaics. Development 125(12): 2263--2271. (Export to RIS)
FlyBase ID	FBrf0103253
Publication Type	Research paper
PubMed ID	9584125
PubMed Abstract	We have developed a 'directed mosaic' system in Drosophila by using the GAL4 system to control the expression of the yeast recombinase, FLP, in a spatial and temporal fashion. By directing FLP expression, we show that it is possible to efficiently and specifically target loss-of-function studies for vital loci to the developmental pathway of interest. A simple F1 adult phenotypic screen demonstrated that most adult tissues can be analyzed with this approach. Using GAL4 lines expressed during oogenesis, we have refined the system to examine the roles of vital loci in the development of the follicular epithelium. We have identified essential genes involved in egg chamber organization, cell migration and cell shape. Further, we have used this technique to gain insights into the role of the Drosophila EGF receptor pathway in establishing the egg axes. Finally, using different UAS-FLP, GAL4 and existing FRT lines, we have built stocks that permit the analysis of approximately 95% of the genome in follicular mosaics.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (17)
	Avic\GFP^Scer\UAS.cYa Avic\GFP^Ubi-p63E.PD csw⁶⁶⁷ csw⁸¹⁷ Dsor1⁷⁵⁶ Ecol\lacZ^AN365 Ecol\lacZ^{Scer\FRT.Act5C.T:Zzzz\nls1} l(1)1070¹⁰⁷⁰ mys⁹⁶⁸ RasGAP1^B2 Scer\FLP1^Scer\UAS.cDa Scer\GAL4^e22c Scer\GAL4^en-e16E Scer\GAL4^T155 sgg^M11 tsl⁶²¹ w^+mC
Constructs (4)
	001298 P{Act5C(FRT.polyA)lacZ.nls1} P{UAS-FLP1.D} UAS::FLP1
Genes (17)
	Avic\GFP bcd csw dl Dsor1 Ecol\lacZ l(1)1070 mys phl RasGAP1 Scer\FLP1 Scer\GAL4 sgg T:Zzzz\nls1 tsl twi w
Insertions (5)
	P{en2.4-GAL4}e16E P{en2.4-GAL4}e22c P{lacZ}AN365 P{UAS-FLP1.D}JD1 P{UAS-FLP1.D}JD2