Open Close
Reference
Citation
Waltzer, L., Bienz, M. (1998). Drosophila CBP represses the transcription factor TCF to antagonize Wingless signalling.  Nature 395(6701): 521--525.
FlyBase ID
FBrf0104794
Publication Type
Research paper
Abstract

T-cell factor (TCF), a high-mobility-group domain protein, is the transcription factor activated by Wnt/Wingless signalling. When signalling occurs, TCF binds to its coactivator, beta-catenin/Armadillo, and stimulates the transcription of the target genes of Wnt/Wingless by binding to TCF-responsive enhancers. Inappropriate activation of TCF in the colon epithelium and other cells leads to cancer. It is therefore desirable for unstimulated cells to have a negative control mechanism to keep TCF inactive. Here we report that Drosophila CREB-binding protein (dCBP) binds to dTCF. dCBP mutants show mild Wingless overactivation phenotypes in various tissues. Consistent with this, dCBP loss-of-function suppresses the effects of armadillo mutation. Moreover, our data show that dCBP acetylates a conserved lysine in the Armadillo-binding domain of dTCF, and that this acetylation lowers the affinity of Armadillo binding to dTCF. Although CBP is a coactivator of other transcription factors, our data show that CBP represses TCF.

PubMed ID
PubMed Central ID
Related Publication(s)
Review

Paper alert.
Miska, 1998, Curr. Opin. Genet. Dev. 8(6): 608 [FBrf0106071]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nature
    Title
    Nature
    Publication Year
    1869-
    ISBN/ISSN
    0028-0836
    Data From Reference
    Alleles (12)
    Genes (12)
    Insertions (2)
    Experimental Tools (1)
    Transgenic Constructs (4)