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From dg27@XXXXXXXXXXXXXXX Thu Sep 17 09:36:54 1998
To: rd120@XXXXXXXXXXXXXXX
Dear Rachel,
I have put everything back together again, which I suppose is one
up on all the Kings horses etc. XX David
The latest two Cam Dps now have stock numbers and appear on our FlyBase
stock list:
4744 In(2LR)DTD99[L]RevB[R], dpp[d-ho] cn[1] bw[1]/CyO = Dp(2;2)Cam17
4745 In(2LR)Pu[LyL]DTD99[R]/CyO = Dp(2;2)Cam18
I want to be sure all of the stocks are in good shape before I send out the
announcement. I will temporarily expand the stocks, since we usually get a
burst of orders whenever we post one of these announcements on
bionet.drosophila.
Look for the 'advertisement' in 2 to 3 weeks.
Thanks for the stocks and helpful comments
Kevin
>The Cam Duplication Kit
>
>A set of large duplications covering >90% of the second chromosome is now
>available from the Bloomington Drosophila Stock Center. These Dp(2;2)
>stocks were generated and donated by David Gubb, Darin Coulson, Siegrun
>Herrmann and John Roote of Cambridge University. These duplications are
>useful in screening for dosage-dependent enhancers and suppressors of
>mutant phenotypes. In addition, because the CAM duplications are nested
>within inverted chromosomal segments, they act as balancer chromosomes for
>the duplicated regions. This makes the CAM duplications ideal for
>recovering (and maintaining) haplo-lethal, or haplo-sterile, mutations.
>
>Short name Stock # Duplicated region
>
>Dp(2;2)Cam1 3392 21E2 to 23D1
>Dp(2;2)Cam2 3394 23D1 to 26C1-2
>Dp(2;2)Cam3 3401 26C1-2 to 29E
>Dp(2;2)Cam4 3403 29E to 32F
>Dp(2;2)Cam5 4521 32F to 35B1-2
>Dp(2;2)Cam6 4518 35B1-2 to 36C
>Dp(2;2)Cam8 2630 36C to 2Lh
>Dp(2;2)Cam10 4520 2Rh to 43A1-2
>Dp(2;2)Cam11 2626 43A1-2 to 47B10-14
>Dp(2;2)Cam13 3391 47B10-14 to 48C
>Dp(2;3)Cam14T 4519 49A to 51EF
>Dp(2;2)Cam17 4744 52D10-E1 to 53F
>Dp(2;2)Cam18 4745 53F to 57C4-6
>Dp(2;2)Cam16 2622 57C4-6 to 60E5-8>
>The genotypes of these duplication stocks are given below as they appear in
>the Bloomington stock list. (Note that the 'Dp(2;2)Cam' chromosome names
>are used for convenience. The full designations of these aberrations
>reflect their construction from recombinants between pairs of pericentric
>inversions.
>
>3392 In(2LR)DTD8[L]S[325R]/CyO, bw[1] = Dp(2;2)Cam1
>3394 In(2LR)DTD24[L]DTD8[R], sp[1]/CyO, bw[1] = Dp(2;2)Cam2
>3401 In(2LR)DTD111[L]DTD24[R], bw[1] sp[1]/CyO, bw[1] = Dp(2;2)Cam3
>3403 In(2LR)DTD107[L]DTD111[R], Dp(1;2)TE23CD, vg[1]/CyO, bw[1] =
>Dp(2;2)Cam4
>4518 In(2LR)S1[L]noc[4R], cn[1] bw[1]/CyO = Dp(2;2)Cam6
>4521 In(2LR)noc[4L]DTD107[R], bw[1] sp[1]/In(2LR)Gla, Gla[1] Bc[1] Egfr[E1]
>ap[*] cn[1] bw[1] = Dp(2;2)Cam5
>2630 In(2LR)C3[L]CH9-25[R], b[1]/In(2L)Cy, In(2R)Cy, al[2] Cy[1] pr[1]
>Bl[1] cn[2] vg[1] c[1] sp[2] = Dp(2;2)Cam8
>4520 In(2LR)TE35B-SZ4[L]noc[4R], (al[1]) dp[ov1] b[1] noc[TE35B] pr[1]
>cn[1] bw[1]/CyO, Df(2L)noc20 = Dp(2;2)Cam10
>2626 (y[*] w[*]); In(2LR)TE35B-226[L]TE35B-4[R], b[1] pr[1] pwn[1]/CyO,
>b[1] rk[1] = Dp(2;2)Cam11
>3391 In(2LR)DTD128[L]TE35B-226[R], Dp(1;2)TE23CD, net[1] dpp[d-ho]/CyO =
>Dp(2;2)Cam13
>4519 Ts(2Lt;3Rt)6r23+Ts(2Rt;3Lt)H24, cn[1]/CyO = Dp(2;3)Cam14T (NB:
>translocation segregants)
4744 In(2LR)DTD99[L]RevB[R], dpp[d-ho] cn[1] bw[1]/CyO = Dp(2;2)Cam17
4745 In(2LR)Pu[LyL]DTD99[R]/CyO = Dp(2;2)Cam18
>2622 In(2LR)lt[G16L]Pu[LyR], b[1] bw[1]/CyO = Dp(2;2)Cam16
>
>This list of stocks is also available from
>http://flybase.bio.indiana.edu/stocks/stock-centers/bloomington/lk/cam-dp-kit
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