From kcook@XXXX Wed Sep 23 21:05:56 1998 Envelope-to: rd120@XXXX Delivery-date: Wed, 23 Sep 1998 21:05:56 +0100 X-Sender: kcook@XXXX X-Mailer: Windows Eudora Light Version 3.0.1 (32) Date: Wed, 23 Sep 1998 15:04:31 -0500 To: flybase-updates@XXXX From: Kevin Cook <kcook@XXXX> Subject: VA228 Mime-Version: 1.0 To: FlyBase updates From: Kevin Cook at the Bloomington Drosophila Stock Center (9/23/98) We recently acquired George Lefevre's line VA228 from Norbert Perrimon. VA228 was listed as both uncl10 and stn10 in Lindsley and Zimm. They cited Lefevre and Watkins (Genetics 113: 869-895, 1986) as the source of this information. This citation was used as a catch-all reference for information about Lefevre lethals that really came from correspondence with Lefevre. VA228 was never mentioned by name in the paper. Currently, FlyBase lists VA228 as a synonym for uncl10. It is not given as a synonym for stnA10, even though the mutation is shown originating with Lefevre. In Perrimon et al. (Genetics 121: 313--331, 1989) where lethals at the base of the X chromosome were described, VA228 was given as an allele of uncl, but not stn. Norbert Perrimon sent the stock as uncl10 and knew nothing about a stnA mutation. I crossed VA228/FM7 females to stnA1/Dp( 1:Y )y+mal106 males and VA228/stnA1 females were viable. They showed no bang-sensitivity, paralysis or uncoordination at room temperature or after an hour at 29 degrees. uncl and stnA are tightly linked and the VA228 stock was maintained by Perrimon as it was received from Lefevre. No attempt was ever made to isolate a uncl mutation away from other linked lethals. I suspect that stnA10 never existed and that there was some problem incorporating Lefevre's notes into Lindsley and Zimm. The phenotypes of uncl and stnA are similar enough to cause confusion. We have chosen to list VA228 as uncl10/FM7 (stock 4734) in our stock list. I believe that Lefevre's correspondence with Dan Lindsley are archived at Cambridge and the source of the mistake might be apparent from examining those notes. Regardless, the gene entries for uncl and stnA should be modified.