Reference Report

Reference
Citation	Maixner, A., Hecker, T.P., Phan, Q.N., Wassarman, D.A. (1998). A screen for mutations that prevent lethality caused by expression of activated Sevenless and Ras1 in the Drosophila embryo. Dev. Genet. 23(4): 347--361. (Export to RIS)
FlyBase ID	FBrf0105879
Publication Type	Research paper
PubMed ID	9883586
PubMed Abstract	Ras1 plays a critical role in receptor tyrosine kinase (RTK) signal transduction pathways that function during Drosophila development. We demonstrate that mis-expression of constitutively active forms of Ras1 (Ras1V12) and the Sevenless (Sev) RTK (SevS11) during embryogenesis causes lethality due to inappropriate activation of RTK/Ras1 signaling pathways. Genetic and molecular data indicate that the rate of SevS11/sev-Ras1V12 lethality is sensitive to the expression level of both transgenes. To identify genes that encode components of RTK/Ras1 signaling pathways or modulators of RNA polymerase II transcription, we took advantage of the dose-sensitivity of the system and screened for second site mutations that would dominantly suppress the lethality. The collection of identified suppressors includes the PR55 subunit of Protein Phosphatase 2A indicating that downstream of Sev and Ras1 this subunit acts as a negative regulator of phosphatase activity. The isolation of mutations in the histone deacetylase RPD3 suggests that it functions as positive regulator of sev enhancer-driven transcription. Finally, the isolation of mutations in the Trithorax group gene devenir and the characterized allelism with the Breathless RTK encoding gene provides evidence for Ras1-mediated regulation of homeotic genes.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Genet.
Title	Developmental Genetics
Publication Year	1979-1999
ISBN/ISSN	0192-253X
Data from Reference
Aberrations (9)
	Df(2L)ast2 Df(2L)JK12 Df(2R)nap9 Df(2R)pk78c Df(3L)vin2 Df(3R)293γ5 Df(3R)red-P52 Df(3R)su(Hw)V Df(3R)urd
Alleles (85)
	aop^ACT.sev ash1² brm¹ brm² btl⁰⁰²⁰⁸ btl⁰⁴⁷²⁷ Dl^03745b eRF1⁰⁵⁶³⁷ fray⁰⁷⁵⁵¹ frc¹⁰⁰⁹⁸ ftz-f1⁰³⁶⁴⁹ InR⁰⁵⁵⁴⁵ kis¹ kis² ksr^XS-2424 ksr^XS-2832 kto¹ l(3)77CDf⁰⁴⁵²¹ l(3)03077⁰³⁰⁷⁷ l(3)03608⁰³⁶⁰⁸ l(3)04704a^04704a l(3)04704b^04704b l(3)04708⁰⁴⁷⁰⁸ l(3)05143⁰⁵¹⁴³ l(3)05820⁰⁵⁸²⁰ l(3)06265⁰⁶²⁶⁵ l(3)06658⁰⁶⁶⁵⁸ l(3)06734⁰⁶⁷³⁴ l(3)06743⁰⁶⁷⁴³ l(3)06948a^06948a l(3)09291⁰⁹²⁹¹ mam^S-176 mor¹ mor² osa¹ osa² osa⁰⁴⁵³⁹ Pc³ Pc⁴ phyl²²⁴⁵ phyl²⁸²⁹ phyl^hs.sev phyl^sev.PC pnt^04263b pnt⁰⁷⁸²⁵ Psa⁰⁶²⁴⁰ Ras85D^e1B Ras85D^E4 Ras85D^N17.hs.2sev Ras85D^V12.sev rl^XS-2275 rl^XS-2442 ro^sev.PI ro^sev.PII Rpd3⁰⁴⁵⁵⁶ RpII15^V RpII140^AS sev^{S11.T:Hsap\MYC} skd¹ skd² sls¹ Sos^e4G SR3-7^S-499 SR3-7^XS-17N SR3-8^S-959 SR3-8^XS-701 sr^06948b Su(Tpl)^S-192 Su(Tpl)^S-705 Taf1^S-625 Taf1^XS-2232 Taf4^S-466 Taf4^XS-793 Taf6^XS-922 TfIIA-S^E32 Trl⁶² trx^unspecified tws⁰²⁴¹⁴ tws^03745a tws⁰⁶⁸⁴⁸ urd² Vha55¹⁵ Vha55¹⁶ vtd³ βggt-I^S-2554
Constructs (7)
	P{sev5} P{sevhs-Ras1^N17} P{sev-phyl} P{Sev-phyl} P{sevRas1.V12} P{sevroI} P{sev-yan^ACT}
Genes (61)
	aop ash1 brm btl Dl eRF1 fray frc ftz-f1 InR kis ksr kto l(3)77CDf l(3)03077 l(3)03608 l(3)04704a l(3)04704b l(3)04708 l(3)05143 l(3)05820 l(3)06240 l(3)06265 l(3)06658 l(3)06734 l(3)06743 l(3)06948a l(3)09291 mam mor osa Pc phyl pnt Ras85D rl ro Rpd3 RpII15 RpII140 sev skd sls Sos sr SR3-7 SR3-8 Su(Pc)37D Su(Tpl) T:Hsap\MYC Taf1 Taf4 Taf6 TfIIA-S Trl trx tws urd Vha55 vtd βggt-I
Insertions (30)
	P{PZ}btl⁰⁰²⁰⁸ P{PZ}btl⁰⁴⁷²⁷ P{PZ}Dl^03745b P{PZ}eRF1⁰⁵⁶³⁷ P{PZ}fray⁰⁷⁵⁵¹ P{PZ}frc¹⁰⁰⁹⁸ P{PZ}ftz-f1⁰³⁶⁴⁹ P{PZ}InR⁰⁵⁵⁴⁵ P{PZ}l(3)77CDf⁰⁴⁵²¹ P{PZ}l(3)03077⁰³⁰⁷⁷ P{PZ}l(3)03608⁰³⁶⁰⁸ P{PZ}l(3)04704a^04704a P{PZ}l(3)04704b^04704b P{PZ}l(3)04708⁰⁴⁷⁰⁸ P{PZ}l(3)05820⁰⁵⁸²⁰ P{PZ}l(3)06265⁰⁶²⁶⁵ P{PZ}l(3)06658⁰⁶⁶⁵⁸ P{PZ}l(3)06734⁰⁶⁷³⁴ P{PZ}l(3)06743⁰⁶⁷⁴³ P{PZ}l(3)06948a^06948a P{PZ}l(3)09291⁰⁹²⁹¹ P{PZ}osa⁰⁴⁵³⁹ P{PZ}pnt^04263b P{PZ}pnt⁰⁷⁸²⁵ P{PZ}Psa⁰⁶²⁴⁰ P{PZ}Rpd3⁰⁴⁵⁵⁶ P{PZ}sr^06948b P{PZ}tws⁰²⁴¹⁴ P{PZ}tws^03745a P{PZ}tws⁰⁶⁸⁴⁸