Reference Report

Reference
Citation	Rorth, P. (1998). Gal4 in the Drosophila female germline. Mech. Dev. 78(1-2): 113--118. (Export to RIS)
FlyBase ID	FBrf0105917
Publication Type	Research paper
PubMed ID	9858703
PubMed Abstract	The modular Gal4 system has proven to be an extremely useful tool for conditional gene expression in Drosophila. One limitation has been the inability of the system to work in the female germline. A modified Gal4 system that works throughout oogenesis is presented here. To achieve germline expression, it was critical to change the basal promoter and 3'-UTR in the Gal4-responsive expression vector (generating UASp). Basal promoters and heterologous 3'-UTRs are often considered neutral, but as shown here, can endow qualitative tissue-specificity to a chimeric transcript. The modified Gal4 system was used to investigate the role of the Drosophila FGF homologue branchless, ligand for the FGF receptor breathless, in border cell migration. FGF signaling guides tracheal cell migration in the embryo. However, misexpression of branchless in the ovary had no effect on border cell migration. Thus border cells and tracheal cells appear to be guided differently.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Mech. Dev.
Title	Mechanisms of Development
Publication Year	1990-
ISBN/ISSN	0925-4773
Data from Reference
Alleles (18)
	bnl^{Scer\UAS.P\T.cRa} Ecol\lacZ^Scer\UAS.cBa Ecol\lacZ^Scer\UAS.cRa Ecol\lacZ^Scer\UAS.cRc Ecol\lacZ^Scer\UAS.cRd Ecol\lacZ^Scer\UAS.cRe Ecol\lacZ^Scer\UAS.cRf Ecol\lacZ^{Scer\UAS.P\T.cRb} Scer\GAL4^arm.PS Scer\GAL4^{nos.UTR.T:Hsim\VP16} Scer\GAL4^otu.PR Scer\GAL4^{otu.T:Hsim\VP16} Scer\GAL4^ptc-559.1 Scer\GAL4^slbo.2.6 Scer\UAS^14x.P\T.K10 slbo⁰¹³¹⁰ TrlBR^cRa w^+mC
Constructs (17)
	P{GAL4::VP16-nos.UTR} P{GAL4-arm.S} P{GAL4-slbo.2.6} P{otu-GAL4::VP16.R} P{otu-GAL4.R} P{UAS-lacZ.B} P{UAS-lacZ.chic.d} P{UAS-lacZ.chic.p} P{UAS-lacZ.hb.m} P{UAS-lacZ.hb.z} P{UAS-lacZ.HZ50} P{UASp} P{UASp-bnl.R} P{UASp-lacZ} P{UAST} pP{UASp} pP{UAST}
Genes (11)
	arm bnl Ecol\lacZ nos otu Scer\GAL4 Scer\UAS slbo T:Hsim\VP16 TrlBR w
Insertions (1)
	P{GawB}ptc^559.1