Reference Report

Reference
Citation	Thor, S., Andersson, S.G., Tomlinson, A., Thomas, J.B. (1999). A LIM-homeodomain combinatorial code for motor-neuron pathway selection. Nature 397(6714): 76--80. (Export to RIS)
FlyBase ID	FBrf0105986
Publication Type	Research paper
PubMed ID	9892357
PubMed Abstract	Different classes of vertebrate motor neuron that innervate distinct muscle targets express unique combinations of LIM-homeodomain transcription factors, suggesting that a combinatorial code of LIM-homeodomain proteins may underlie the control of motor-neuron pathway selection. Studies of LIM-homeodomain genes in mouse, Drosophila melanogaster and Caenorhabditis elegans have revealed functions of these genes in neuronal survival, axon guidance, neurotransmitter expression and neuronal function, but, to our knowledge, none of these studies have addressed the issue of a functional code. Here we study two members of this gene family in Drosophila, namely lim3, the homologue of the vertebrate Lhx3 and Lhx4 genes, and islet, the homologue of the vertebrate Isl1 and Is12 genes. We show that Drosophila lim3 is expressed by a specific subset of islet-expressing motor neurons and that mutating or misexpressing lim3 switches motor-neuron projections predictably. Our results provide evidence that lim3 and islet constitute a combinatorial code that generates distinct motor-neuron identities.
DOI
Related Publication(s)
Review	Paper alert. Toyama, 1999, Curr. Opin. Cell Biol. 11(2): 167 [FBrf0108037] Paper alert. Toyama, 1999, Curr. Opin. Genet. Dev. 9(2): 124 [FBrf0108370] Paper alert. Anonymous, 1999, Curr. Opin. Neurobiol. 9(2): 144 [FBrf0112206]
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Nature
Title	Nature
Publication Year	1869-
ISBN/ISSN	0028-0836
Data from Reference
Aberrations (3)
	Df(2L)E71 Df(2L)OD15 Df(2L)TW130
Alleles (10)
	Avic\GFP^{Scer\UAS.T:Btau\MAPT,T:Hsap\MYC} Btau\MAPT^{isl.H.T:Hsap\MYC} Ecol\lacZ^{ap.C.T:Btau\MAPT} Lim3¹ Lim3⁶ Lim3⁷ Lim3^Scer\UAS.cTa Lim3^{T:Btau\MAPT,T:Hsap\MYC} Lim3^T:Ecol\lacZ Scer\GAL4^ftz.ng
Constructs (7)
	P{apC-tau-lacZ} P{GAL4-ftz.ng} P{islH-tau-myc} P{Lim3.lacZ.T} P{Lim3.tau-myc.T} P{UAS-btau::GFP.T:Myc} P{UAS-lim3.T}
Genes (25)
	Acn amd ap Avic\GFP Btau\MAPT Catsup CG10470 CG10492 CG10561 cub Ddc Ecol\lacZ hk l(2)37Ab l(2)37Ac l(2)37Bi l(2)37Bj Lim3 mib2 Rpn3 Scer\GAL4 T:Btau\MAPT T:Ecol\lacZ T:Hsap\MYC tup