Reference Report

Reference
Citation	Ishimaru, S., Williams, R., Clark, E., Hanafusa, H., Gaul, U. (1999). Activation of the Drosophila C3G leads to cell fate changes and overproliferation during development, mediated by the RAS-MAPK pathway and RAP1. EMBO J. 18(1): 145--155. (Export to RIS)
FlyBase ID	FBrf0106038
Publication Type	Research paper
External Crossreferences
PubMed ID	9878058
PubMed Abstract	The cellular signal transduction pathways by which C3G, a RAS family guanine nucleotide exchange factor, mediates v-crk transformation are not well understood. Here we report the identification of Drosophila C3G, which, like its human cognate, specifically binds to CRK but not DRK/GRB2 adaptor molecules. During Drosophila development, constitutive membrane binding of C3G, which also occurs during v-crk transformation, results in cell fate changes and overproliferation, mimicking overactivity of the RAS-MAPK pathway. The effects of C3G overactivity can be suppressed by reducing the gene dose of components of the RAS-MAPK pathway and of RAP1. These findings provide the first in vivo evidence that membrane localization of C3G can trigger activation of RAP1 and RAS resulting in the activation of MAPK, one of the hallmarks of v-crk transformation previously thought to be mediated through activation of SOS.
BIOSIS ID	1999.57952
Zoological Record ID
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Text of Personal Communication
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Related Publications
Review	Paper alert. Ridley, 1999, Curr. Opin. Genet. Dev. 9(2): 119--120 [FBrf0108366]
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
ISBN
Parent Publication
Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Editors
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Aberrations (1)
	Df(3L)R-G7
Alleles (15)
	C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} C3G^{Scer\UAS.T:Hsap\MYC} C3G^{Δ.Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} drk^e0A ksr^S-638 R^rvB1 Ras85D^e1B Ras85D^N17.Scer\UAS Ras85D^V12.Scer\UAS rl^x-3-30 Scer\GAL4^dpp.blk1 Scer\GAL4^GMR.PF Scer\GAL4^unspecified Sos^e4G w^+mC
Constructs (7)
	P{GAL4-dpp.blk1} P{GAL4-ninaE.GMR} P{UAS-C3G.T:Myc.T:Myr3} P{UAS-C3G.T:Myc} P{UAS-C3G.Δ.T:Myc.T:Myr3} P{UAS-Ras85D.N17} P{UAS-Ras85D.V12}
Genes (12)
	C3G drk elav ksr R Ras85D rl Scer\GAL4 Sos T:Ggal\Myr3 T:Hsap\MYC w
Transcripts (1)
	C3G-XR