Reference Report

Reference
Citation	Adachi-Yamada, T., Nakamura, M., Irie, K., Tomoyasu, Y., Sano, Y., Mori, E., Goto, S., Ueno, N., Nishida, Y., Matsumoto, K. (1999). p38 mitogen-activated protein kinase can be involved in transforming growth factor beta superfamily signal transduction in Drosophila wing morphogenesis. Mol. Cell. Biol. 19(3): 2322--2329. (Export to RIS)
FlyBase ID	FBrf0106118
Publication Type	Research paper
PubMed ID	10022918
PubMed Abstract	p38 mitogen-activated protein kinase (p38) has been extensively studied as a stress-responsive kinase, but its role in development remains unknown. The fruit fly, Drosophila melanogaster, has two p38 genes, D-p38a and D-p38b. To elucidate the developmental function of the Drosophila p38's, we used various genetic and pharmacological manipulations to interfere with their functions: expression of a dominant-negative form of D-p38b, expression of antisense D-p38b RNA, reduction of the D-p38 gene dosage, and treatment with the p38 inhibitor SB203580. Expression of a dominant-negative D-p38b in the wing imaginal disc caused a decapentaplegic (dpp)-like phenotype and enhanced the phenotype of a dpp mutant. Dpp is a secretory ligand belonging to the transforming growth factor beta superfamily which triggers various morphogenetic processes through interaction with the receptor Thick veins (Tkv). Inhibition of D-p38b function also caused the suppression of the wing phenotype induced by constitutively active Tkv (TkvCA). Mosaic analysis revealed that D-p38b regulates the Tkv-dependent transcription of the optomotor-blind (omb) gene in non-Dpp-producing cells, indicating that the site of D-p38b action is downstream of Tkv. Furthermore, forced expression of TkvCA induced an increase in the phosphorylated active form(s) of D-p38(s). These results demonstrate that p38, in addition to its role as a transducer of emergency stress signaling, may function to modulate Dpp signaling.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Aberrations (4)
	Df(2L)b82a2 Df(2L)C28 Df(2L)flp147E Df(3R)crb-F89-4
Alleles (21)
	bi^omb-1 bsk^DN.Scer\UAS dpp^d5 dpp^d8 dpp^hr27 dpp^hr56 Ecol\lacZ^bi-Pol-1 Egfr^E1 N^Ax-1 p38b^a.Scer\UAS p38b^DN.Scer\UAS p38b^Scer\UAS.cAa Scer\GAL4^32B Scer\GAL4^69B Scer\GAL4^71B Scer\GAL4^Act5C.PI Scer\GAL4^{Scer\FRT.Act5C} tkv⁷ tkv⁴²⁷ tkv^CA.Scer\UAS w^+mC
Constructs (7)
	P{Act5C-GAL4} P{AyGAL4} P{UAS-bsk.DN} P{UAS-p38b.a} P{UAS-p38b.A} P{UAS-p38b.DN} P{UAS-tkv.CA}
Genes (14)
	bi bsk dpp Ecol\lacZ Egfr kuz l(2)34Db lace N p38b Scer\GAL4 tam tkv w
Insertions (4)
	P{GawB}32B P{GawB}69B P{GawB}71B P{lacW}bi^Pol-1