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Citation
Toba, G., Ohsako, T., Miyata, N., Ohtsuka, T., Seong, K.H., Aigaki, T. (1999). The gene search system. A method for efficient detection and rapid molecular identification of genes in Drosophila melanogaster.  Genetics 151(2): 725--737.
FlyBase ID
FBrf0107369
Publication Type
Research paper
Abstract
We have constructed a P-element-based gene search vector for efficient detection of genes in Drosophila melanogaster. The vector contains two copies of the upstream activating sequence (UAS) enhancer adjacent to a core promoter, one copy near the terminal inverted repeats at each end of the vector, and oriented to direct transcription outward. Genes were detected on the basis of phenotypic changes caused by GAL4-dependent forced expression of vector-flanking DNA, and the transcripts were identified with reverse transcriptase PCR (RT-PCR) using the vector-specific primer and followed by direct sequencing. The system had a greater sensitivity than those already in use for gain-of-function screening: 64% of the vector insertion lines (394/613) showed phenotypes with forced expression of vector-flanking DNA, such as lethality or defects in adult structure. Molecular analysis of 170 randomly selected insertions with forced expression phenotypes revealed that 21% matched the sequences of cloned genes, and 18% matched reported expressed sequence tags (ESTs). Of the insertions in cloned genes, 83% were upstream of the protein-coding region. We discovered two new genes that showed sequence similarity to human genes, Ras-related protein 2 and microsomal glutathione S-transferase. The system can be useful as a tool for the functional mapping of the Drosophila genome.
PubMed ID
PubMed Central ID
PMC1460511 (PMC) (EuropePMC)
DOI
Related Publication(s)
Personal communication to FlyBase
Re: GS2038.
Aigaki, 2007.3.19, Re: GS2038. [FBrf0199125]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Alleles (73)
    Genes (65)
    Datasets (1)
    Molecular Constructs (1)
    Molecular Segments (8)
    Natural transposons (1)
    Insertions (106)
    Experimental Tools (1)
    Transgenic Constructs (5)