Reference Report

Reference
Citation	Hayashi, S., Yamaguchi, M. (1999). Kinase-independent activity of Cdc2/Cyclin A prevents the S phase in the Drosophila cell cycle. Genes Cells 4(2): 111--122. (Export to RIS)
FlyBase ID	FBrf0108196
Publication Type	Research paper
PubMed ID	10320477
PubMed Abstract	The Cdc2-dependent inhibition of S phase is required in G2 for the correct ordering of the S and M phases in yeasts and Drosophila. This function of Cdc2 has been ascribed to its ability to phosphorylate replication factors to prevent the assembly of a preinitiation complex at the origin of replication. Whether this is the sole mechanism of S phase inhibition by Cdc2 in higher metazoans is not known because the pleiotropic functions of this essential cell cycle regulator make genetic analysis difficult.We show that Cdc2 co-expressed with Cyclin A inhibits the S phase in Drosophila salivary glands and diploid abdominal histoblasts. A kinase defective mutant of Cdc2 failed to promote mitosis, but was still able to inhibit the S phase with the same efficiency as the wild-type protein. In addition, Cdc2 and Cyclin A cooperatively inhibit transcriptional activation by the essential S phase regulator E2F. Cdc2 binds to E2F in vitro, and post-transcriptionally promotes its accumulation in vivo. Furthermore, the inhibitory effect of Cdc2 on S phase is overridden by E2F.The inhibition of S phase by Cdc2 is achieved in part by a kinase-independent mechanism, which is likely to be mediated by the inhibition of E2F.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Cells
Title	Genes to cells : devoted to molecular & cellular mechanisms
Publication Year	1996-
ISBN/ISSN	1356-9597
Data from Reference
Aberrations (1)
	Df(2L)J27
Alleles (16)
	cdc2^B47 cdc2^E1-24 cdc2^Scer\UAS.cHa CycA^Scer\UAS.cHa E2f^Act5C.PD E2f^hs.PA Ecol\CAT^{mus209.5'-116} Ecol\CAT^{mus209.5'-168} Ecol\CAT^{mus209.E2FmutI&II} Scer\GAL4^Act5C.PHb Scer\GAL4^hs.PB Scer\GAL4^l(1)BP-1 Scer\GAL4^P127 w^+mC Xlae\cdc2^{K33R.Scer\UAS} Xlae\cdc2^Scer\UAS.cHa
Constructs (6)
	P{GAL4-Hsp70.PB} P{hsp70-E2F} P{UAS-cdc2.H} P{UAS-CycA.H} P{UAS-Xlae\cdc2.H} P{UAS-Xlae\cdc2.K33R}
Genes (8)
	cdc2 CycA E2f Ecol\CAT mus209 Scer\GAL4 w Xlae\cdc2
Insertions (2)
	P{GAL4}P127 P{GawB}l(1)BP¹