Reference Report

Reference
Citation	Liu, Y., Montell, D.J. (1999). Identification of mutations that cause cell migration defects in mosaic clones. Development 126(9): 1869--1878. (Export to RIS)
FlyBase ID	FBrf0108422
Publication Type	Research paper
PubMed ID	10101121
PubMed Abstract	Cell movement is an important feature of animal development, wound healing and tumor metastasis; however, the mechanisms underlying cell motility remain to be elucidated. To further our understanding, it would be useful to identify all of the proteins that are essential for a cell to migrate, yet such information is not currently available for any cell type. We have carried out a screen for mutations affecting border cell migration in Drosophila. Mutations that cause defects in mosaic clones were identified, so that genes that are also required for viability could be detected. From 6000 mutagenized lines, 20 mutations on chromosome 2R were isolated that cause defects in border cell position. One of the mutations was dominant while all of the recessive mutations appeared to be homozygous lethal. This lethality was used to place the mutations into 16 complementation groups. Many of the mutations failed to complement cytologically characterized deficiencies, allowing their rapid mapping. Mutations in three loci altered expression of a marker gene in the border cells, whereas the remaining mutations did not. One mutation, which caused production of supernumerary border cells, was found to disrupt the costal-2 locus, indicating a role for Hedgehog signaling in border cell development. This screen identified many new loci required for border cell migration and our results suggest that this is a useful approach for elucidating the mechanisms involved in cell motility.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (28)
	31B8^31B8 cos^52B7 cos^k16101 cos^unspecified Ecol\lacZ⁰³⁰⁵⁰ Ecol\lacZ⁶³⁵⁶ Ecol\lacZ^ken-02970 jing^22F3 jing^31E6 jing^47H6 l(2)2D8^2D8 l(2)2D8^9B9 l(2)3A2^3A2 l(2)11B9^11B9 l(2)11B9^23A9 l(2)12A9^12A9 l(2)17E1^17E1 l(2)18E4^18E4 l(2)46F4^46F4 l(2)50F7^50F7 l(2)61E5^61E5 l(2)65C2^65C2 par-1^27C1 Scer\FLP1^Scer\UAS.cDa Scer\GAL4^T155 slbo^5D4 slbo^e7b zip^17F1
Constructs (1)
	P{UAS-FLP1.D}
Genes (21)
	31B8 cos Ecol\lacZ Fas3 jing l(2)2D8 l(2)3A2 l(2)11B9 l(2)12A9 l(2)17E1 l(2)18E4 l(2)46F4 l(2)50F7 l(2)61E5 l(2)65C2 par-1 Scer\FLP1 Scer\GAL4 shg slbo zip
Insertions (5)
	P{GawB}T155 P{lacW}cos^k16101 P{PZ}6356 P{PZ}ken⁰²⁹⁷⁰ P{PZ}l(2)03050⁰³⁰⁵⁰