Reference Report

Reference
Citation	Niimi, T., Seimiya, M., Kloter, U., Flister, S., Gehring, W.J. (1999). Direct regulatory interaction of the eyeless protein with an eye-specific enhancer in the sine oculis gene during eye induction in Drosophila. Development 126(10): 2253--2260. (Export to RIS)
FlyBase ID	FBrf0108431
Publication Type	Research paper
PubMed ID	10207149
PubMed Abstract	The Pax-6 gene encodes a transcription factor with two DNA-binding domains, a paired and a homeodomain, and is expressed during eye morphogenesis and development of the nervous system. Pax-6 homologs have been isolated from a wide variety of organisms ranging from flatworms to humans. Since loss-of-function mutants in insects and mammals lead to an eyeless phenotype and Pax-6 orthologs from distantly related species are capable of inducing ectopic eyes in Drosophila, we have proposed that Pax-6 is a universal master control gene for eye morphogenesis. To determine the extent of evolutionary conservation of the eye morphogenetic pathway, we have begun to identify subordinate target genes of Pax-6. Previously we have shown that expression of two genes, sine oculis (so) and eyes absent (eya), is induced by eyeless (ey), the Pax-6 homolog of Drosophila. Here we present evidence from ectopic expression studies in transgenic flies, from transcription activation studies in yeast, and from gel shift assays in vitro that the EY protein activates transcription of sine oculis by direct interaction with an eye-specific enhancer in the long intron of the so gene.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (8)
	Ecol\lacZ^so-7 Ecol\lacZ^so.5 Ecol\lacZ^so.10 ey^hs.PH ey^Scer\UAS.cHa Scer\GAL4^dpp.blk1 so¹ toy^Scer\UAS.cCa
Constructs (6)
	P{GAL4-dpp.blk1} P{hs-ey} P{so-lacZ.5} P{so-lacZ.10} P{UAS-ey.H} P{UAS-toy.C}
Genes (5)
	Ecol\lacZ ey Scer\GAL4 so toy
Insertions (1)
	P{A92}so⁷
Sequence features (2)
	so_so5 so_so10