Personal communication from: Ulrich Schaefer, Herbert Jackle, Yuchun He, Hugo Bellen, Todd Laverty and Gerry Rubin
To: Bloomington Drosophila Stock Center
Subject: Goettingen lethals - set 2
Dated: 15 June 1999
Background: The lethal and semi-lethal alleles and P{lacW} insertions reported here were generated in the laboratory of
Herbert Jackle (Goettingen) in a screen for lethal P{lacW} hops into the X chromosome. In situ hybridizations to map the
P{lacW} insertions were carried out in the laboratory of Hugo Bellen (Houston). Hybridized chromosomes were read by
both the Bellen lab and by Todd Laverty (Rubin lab, Berkeley). The assumption that lethality in single insertion lines is
caused by the insertion has not been verified. lacZ expression patterns for these lines will be available from FlyView.
This communication includes revised insertion sites for some of the lines included in FBrf0107552, followed by new
alleles and transposon insertions.
Alleles with revised insertion sites (alleles presumed to be caused by P{lacW} insertions):
Original Revised
l(1)G0028[G0028] 11B3-6 12E1-4
l(1)G0030[G0030] 5D3-6 6A
l(1)G0035[G0035] 6F1-2 6E4-5
l(1)G0037[G0037] 1B7-8 1C
l(1)G0040[G0040] 8B1-4 10C1-2
l(1)G0043[G0043] 6F1-2 6F
l(1)G0054[G0054] 3F4-5 3F1-5
l(1)G0056[G0056] 9E3-4 9E1-2
l(1)G0057[G0057] 13F1-2 13E8-15
l(1)G0061[G0061] 9E3-4 5E1-2
l(1)G0063[G0063] 5E1-2 5E1-7
l(1)G0066[G0066] 2C1-2 2C
l(1)G0067[G0067] 8E3-4 8B1-4
l(1)G0071[G0071] 7E5-6 7E3-11
l(1)G0072[G0072] 9C3-4 9F1-4
l(1)G0074[G0074] 16D1-2 16C1-2
l(1)G0075[G0075] 9D 10A4-9
Actn[G0077] 2C1-2 2C
l(1)G0078[G0078] 11D1-2 11E (multi-insert line, no change in other insertion site)
l(1)G0079[G0079] 5C3-6 5C3-9
l(1)G0081[G0081] 4B1-2 4B
l(1)G0083[G0083] 9F1-6 9F
l(1)G0104[G0104] 12D 12C
l(1)G0105[G0105] 1E3-4 1F1-2
l(1)G0115[G0115] 1C2-3 1C1-3
l(1)G0117[G0117] 5C1-2, 5D1-2 5C3-9 (i.e., no longer judged to be a multi-insert line)
l(1)G0118[G0118] 6E1-2 6E1-5
l(1)G0127[G0127] 9E1-2 9E
l(1)G0130[G0130] 2B1-2 2B1-4
Revised transposon insertion conclusions (from multi-insert lines):
Original Revision
P{lacW}G0078a 11D1-2 11E
P{lacW}G0117a 5C1-2 eliminate
P{lacW}G0117b 5D1-2 eliminate
New alleles (presumed to be caused by insertion of P{lacW}:
Allele Insertion site
l(1)G0003[G0003] 17C
l(1)G0004[G0004] 19C
l(1)G0012[G0012] 2A1-2
l(1)G0013[G0013] 18D1-2
l(1)G0014[G0014] 2C1-2
l(1)G0020[G0020] 8B1-4
l(1)G0022[G0022] semi-lethal 13F1-2
l(1)G0024[G0024] 3B
l(1)G0025[G0025] 5D1-2
l(1)G0027[G0027] 13E7-15
l(1)G0036[G0036] semi-lethal 6F1-2
l(1)G0041[G0041] 18D1-4
l(1)G0044[G0044] 2B1-4
l(1)G0046[G0046] ? -- multi-insert line: 3A1-4, 12F1-5
l(1)G0060[G0060] 11B1-4
l(1)G0062[G0062] 19A1-2
l(1)G0065[G0065] 10B1-2
l(1)G0070[G0070] 17C1-2
l(1)G0076[G0076] 9E7-8
l(1)G0086[G0086] 19E
l(1)G0090[G0090] 7A1-5
l(1)G0092[G0092] ? -- multi-insert line: 17C1-4, 17D1-4
l(1)G0094[G0094] semi-lethal ? -- multi-insert line: 4D1-2, 9E3-4
l(1)G0097[G0097] semi-lethal ? -- multi-insert line: 4E, 9C1-2, 12F1-3
l(1)G0099[G0099] 7F1-4
l(1)G0102[G0102] 10E1-4
l(1)G0103[G0103] 12E1-4
l(1)G0111[G0111] 10E
l(1)G0112[G0112] semi-lethal 8F4-7
l(1)G0116[G0116] 14F1-3
l(1)G0120[G0120] 18F1-2
l(1)G0121[G0121] semi-lethal 12D1-2
l(1)G0123[G0123] semi-lethal 13E1-6
l(1)G0124[G0124] 11B-C
l(1)G0125[G0125] 19E
l(1)G0126[G0126] semi-lethal 9F1-2
l(1)G0128[G0128] semi-lethal 14B1-4
l(1)G0137[G0137] ? -- multi-insert line: 8F1-2, 9A1-3
l(1)G0143[G0143] 16B1-3
l(1)G0144[G0144] 2F
l(1)G0147[G0147] ? -- multi-insert line: 3A3, 18C1-2
l(1)G0150[G0150] 18F
l(1)G0153[G0153] 4C13-16
l(1)G0154[G0154] 7D14-21
l(1)G0156[G0156] 18D1-4
l(1)G0161[G0161] ? -- multi-insert line: 7E2-3, 7E5-8
l(1)G0164[G0164] 13E
l(1)G0165[G0165] 17B1-C4
l(1)G0168[G0168] 13D1-2
l(1)G0169[G0169] 10B1-2
l(1)G0174[G0174] 3E
l(1)G0177[G0177] 5C5-6
l(1)G0179[G0179] semi-lethal 19F
l(1)G0186[G0186] 12D-E2
l(1)G0191[G0191] 12C
l(1)G0193[G0193] 7D1-3
l(1)G0196[G0196] 9E1-4
l(1)G0198[G0198] 16C1-2
l(1)G0199[G0199] ? -- multi-insert line: 7D10-14, 18D10-E
l(1)G0200[G0200] 8E1-4
l(1)G0201[G0201] 13C
l(1)G0202[G0202] semi-lethal ? -- multi-insert line: 10C1-2, 12B
l(1)G0203[G0203] semi-lethal 7E5-6
l(1)G0205[G0205] semi-lethal 17C-D
l(1)G0207[G0207] 4C11-14
l(1)G0209[G0209] 18E-F
l(1)G0211[G0211] 3A1-4
l(1)G0212[G0212] 14B5-8
l(1)G0216[G0216] 7C
l(1)G0219[G0219] 7E4-8
l(1)G0220[G0220] semi-lethal 2B13-C2
l(1)G0221[G0221] 14C
l(1)G0223[G0223] 18F
l(1)G0228[G0228] 7E3-6
l(1)G0229[G0229] semi-lethal 13C1-2
l(1)G0233[G0233] 7D3-5
l(1)G0237[G0237] 10C1-2
l(1)G0239[G0239] 13E13-F2
l(1)G0241[G0241] 10D1-3
l(1)G0244[G0244] ? -- multi-insert line: 3A1-4, 4D1-2
l(1)G0245[G0245] 5C5-8
l(1)G0249[G0249] 8E1-4
l(1)G0250[G0250] semi-lethal 8E1-8
l(1)G0251[G0251] semi-lethal 3A3-4
l(1)G0252[G0252] semi-lethal 11C
l(1)G0254[G0254] 6C
l(1)G0259[G0259] 1B1-2
l(1)G0260[G0260] semi-lethal 14B3-8
l(1)G0262[G0262] 13F1-2
l(1)G0263[G0263] 3B1-2
l(1)G0266[G0266] 18D5-8
l(1)G0267[G0267] 10B3-10
l(1)G0268[G0268] ? -- multi-insert line: 2B13-18, 3A1-3
l(1)G0270[G0270] 9E4-10
l(1)G0271[G0271] 3A3-4
l(1)G0272[G0272] 14C3-8
l(1)G0276[G0276] 10B14-17
l(1)G0277[G0277] 12A1-2
l(1)G0278[G0278] 13B5-9
l(1)G0282[G0282] semi-lethal 18E-F
l(1)G0283[G0283] 18D5-8
l(1)G0284[G0284] 2B1-8
l(1)G0286[G0286] 8F
l(1)G0287[G0287] 5E-F
l(1)G0290[G0290] 6C4-8
l(1)G0293[G0293] 4B1-4
l(1)G0296[G0296] 1E
l(1)G0298[G0298] 11C
l(1)G0301[G0301] semi-lethal 2B
l(1)G0307[G0307] ? -- multi-insert line: 7C7-9, 12F1-4
l(1)G0309[G0309] 13F1-2
l(1)G0310[G0310] 2D
l(1)G0311[G0311] ? -- multi-insert line: 3A1-4, 18C1-4
l(1)G0312[G0312] 5A
l(1)G0315[G0315] ? -- multi-insert line: 3A1-4, 13F1-4
l(1)G0317[G0317] 6F1-4
l(1)G0318[G0318] 2B1-8
l(1)G0323[G0323] 8B1-4
l(1)G0324[G0324] semi-lethal ? -- multi-insert line: 3A1-4, 9E3-4
l(1)G0329[G0329] 1B7-10
l(1)G0330[G0330] 5C5-8
l(1)G0332[G0332] 7E
l(1)G0345[G0345] 5F1-3
l(1)G0346[G0346] semi-lethal 8E1-4
l(1)G0355[G0355] semi-lethal 2C1-2
l(1)G0360[G0360] 2C7-D4
l(1)G0363[G0363] semi-lethal ? -- multi-insert line: 18B1-4, 19E1-2
l(1)G0384[G0384] 5A5-9
l(1)G0394[G0394] semi-lethal 12C4-5
l(1)G0395[G0395] semi-lethal 13E1-4
l(1)G0398[G0398] semi-lethal 12C
l(1)G0409[G0409] 18D5-8
l(1)G0423[G0423] semi-lethal ? -- multi-insert line: 2C, 9E1-4
l(1)G0427[G0427] 18F
l(1)G0428[G0428] 19E
New transposon insertions (from multi-insert lines):
P{lacW}G0046a 3A1-4
P{lacW}G0046b 12F1-5
P{lacW}G0092a 17C1-4
P{lacW}G0092b 17D1-4
P{lacW}G0094a 4D1-2
P{lacW}G0094b 9E3-4
P{lacW}G0097a 4E
P{lacW}G0097b 9C1-2
P{lacW}G0097c 12F1-3
P{lacW}G0137a 8F1-2
P{lacW}G0137b 9A1-3
P{lacW}G0147a 3A3
P{lacW}G0147b 18C1-2
P{lacW}G0161a 7E2-3
P{lacW}G0161b 7E5-8
P{lacW}G0199a 7D10-14
P{lacW}G0199b 18D10-E
P{lacW}G0202a 10C1-2
P{lacW}G0202b 12B
P{lacW}G0244a 4D1-2
P{lacW}G0244b 3A1-4
P{lacW}G0268a 2B13-18
P{lacW}G0268b 3A1-3
P{lacW}G0307a 7C7-9
P{lacW}G0307b 12F1-4
P{lacW}G0311a 3A1-4
P{lacW}G0311b 18C1-4
P{lacW}G0315a 3A1-4
P{lacW}G0315b 13F1-4
P{lacW}G0324a 3A1-4
P{lacW}G0324b 9E3-4
P{lacW}G0363a 18B1-4
P{lacW}G0363b 19E1-2
P{lacW}G0423a 2C
P{lacW}G0423b 9E1-4