Reference Report

Reference
Citation	Bellaiche, Y., Mogila, V., Perrimon, N. (1999). I-SceI endonuclease, a new tool for studying DNA double-strand break repair mechanisms in Drosophila. Genetics 152(3): 1037--1044. (Export to RIS)
FlyBase ID	FBrf0109325
Publication Type	Research paper
PubMed ID	10388822
PubMed Abstract	As a step toward the development of a homologous recombination system in Drosophila, we have developed a methodology to target double-strand breaks (DSBs) to a specific position in the Drosophila genome. This method uses the mitochondrial endonuclease I-SceI that recognizes and cuts an 18-bp restriction site. We find that >6% of the progeny derived from males that carry a marker gene bordered by two I-SceI sites and that express I-SceI in their germ line lose the marker gene. Southern blot analysis and sequencing of the regions surrounding the I-SceI sites revealed that in the majority of the cases, the introduction of DSBs at the I-SceI sites resulted in the complete deletion of the marker gene; the other events were associated with partial deletion of the marker gene. We discuss a number of applications for this novel technique, in particular its use to study DSB repair mechanisms.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (9)
	l(3)SCEI-1^SCEI-1 l(3)SCEI-2^SCEI-2 ry^+t7.2 Scer\SCEI^{βTub85D.T:SV40\nls2} w^Δ.XEN y^+mDint2 y^SCEI.P1 y^SCEI.P2 y^SCEI.P3
Constructs (5)
	P{3'FRT+SceI(y⁺,w^-)} P{FRT+SceI(y⁺,w^-)} P{y^SCEI.P1,w^Δ.XEN} P{y^SCEI.P2,w^Δ.XEN} P{βTub85D-Scer\SCEI.nls}
Genes (8)
	l(3)SCEI-1 l(3)SCEI-2 ry Scer\SCEI Scer\SceI.RS T:SV40\nls2 w y
Insertions (2)
	P{βTub85D-Scer\SCEI.nls}l(3)SCEI-1^SCEI-1 P{βTub85D-Scer\SCEI.nls}l(3)SCEI-2^SCEI-2