Reference Report

Reference
Citation	Apidianakis, Y., Nagel, A.C., Chalkiadaki, A., Preiss, A., Delidakis, C. (1999). Overexpression of the m4 and malpha genes of the E(spl)-complex antagonizes notch mediated lateral inhibition. Mech. Dev. 86(1,2): 39--50. (Export to RIS)
FlyBase ID	FBrf0111325
Publication Type	Research paper
PubMed ID	10446264
PubMed Abstract	Intercellular signalling mediated by Notch proteins is crucial to many cell fate decisions in metazoans. Its profound effects on cell fate and proliferation require that a complex set of responses involving positive and negative signal transducers be orchestrated around each instance of signalling. In Drosophila the basic-helix-loop-helix (bHLH) repressor encoding genes of the E(spl) locus are induced by Notch signalling and mediate some of its effects, such as suppression of neural fate. Here we report on a novel family of Notch responsive genes, whose products appear to act as antagonists of the Notch signal in the process of adult sensory organ precursor singularization. They, too, reside in the E(spl) locus and comprise transcription units E(spl) m4 and E(spl) malpha. Overexpression of these genes causes downregulation of E(spl) bHLH expression accompanied by cell autonomous overcommitment of sensory organ precursors and tufting of bristles. Interestingly, negative regulation of the Notch pathway by overexpression of E(spl) m4 and malpha is specific to the process of sensory organ precursor singularization and does not impinge on other instances of Notch signalling.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mech. Dev.
Title	Mechanisms of Development
Publication Year	1990-
ISBN/ISSN	0925-4773
Data from Reference
Aberrations (5)
	Df(1)sc10-1 Df(3R)Dl-BX6 Df(3R)gro^b32.2 Df(3R)Pr4 Dp(1;2)N⁺
Alleles (42)
	bib² Brd^unspecified dx^unspecified E(spl)m4-BFM^Scer\UAS.cAa E(spl)m4-BFM^{Scer\UAS.T:Avic\GFP} E(spl)m7-HLH^{Scer\UAS.cdCa} E(spl)m8-HLH^Scer\UAS.cLa E(spl)mα-BFM^Scer\UAS.cAa E(spl)mβ-HLH^{Scer\UAS.cdCa} E(spl)mγ-HLH^Scer\UAS.cLa E(spl)mδ-HLH^{Scer\UAS.cdCa} Ecol\lacZ^{E(spl).-1166+87} Ecol\lacZ^neur-A101 Ecol\lacZ^Scer\UAS.cUa Ecol\lacZ^Ubx.PdC Ecol\lacZ^vg.int2.1 Ecol\lacZ^wg-en11 H¹ H² H^Scer\UAS.cMa l(1)sc^Scer\UAS.cHa mamⁱ⁵¹¹ N^264-40 N^Ax-1 N^Ax-M1 N^{RICN.Scer\UAS} neur^A101 Ppyr\LUC^E(spl).PA Ppyr\LUC^HLHmγ.PA Scer\GAL4^32B Scer\GAL4^455.2 Scer\GAL4^ap-md544 Scer\GAL4^bi-md653 Scer\GAL4^MtnA.PL Scer\GAL4^pnr-MD237 Scer\GAL4^ptc-559.1 Scer\GAL4^sca-T3 Scer\GAL4^Scer\FRT.Ubx Scer\GAL4^Ubx.PdC Ser^RX82 Su(H)^MtnA.PA w^+mC
Constructs (15)
	P{GAL4-lacZ-Ubx(FRT.f⁺).dC} P{GAL4-lacZ-Ubx(-FRT).dC} P{m8-lacZ} P{UAS-E(spl).L} P{UAS-E(spl)mβ} P{UAS-H.M} P{UAS-HLHmγ.L} P{UAS-l(1)sc.LSC} P{UAS-l(1)sc.ΔB} P{UAS-lacZ.U} P{UAS-m4.A} P{UAS-m4.GFP} P{UAS-mα.A} P{UAS-mδ} P{vg(D/V)-lacZ}
Genes (25)
	bib Brd dx E(spl)m4-BFM E(spl)m7-HLH E(spl)m8-HLH E(spl)mα-BFM E(spl)mβ-HLH E(spl)mγ-HLH E(spl)mδ-HLH Ecol\lacZ elav H l(1)sc mam N neur Ppyr\LUC pros Scer\GAL4 Ser Su(H) T:Avic\GFP Tom w
Insertions (9)
	P{en1}wg^en11 P{GawB}32B P{GawB}455.2 P{GawB}ap^md544 P{GawB}bi^md653 P{GawB}pnr^MD237 P{GawB}ptc^559.1 P{GawB}sca^T3 P{lArB}neur^A101
Natural transposons (1)
	P-element