|Citation||Ashburner, M., Misra, S., Roote, J., Lewis, S.E., Blazej, R., Davis, T., Doyle, C., Galle, R., George, R., Harris, N., Hartzell, G., Harvey, D., Hong, L., Houston, K., Hoskins, R., Johnson, G., Martin, C., Moshrefi, A., Palazzolo, M., Reese, M.G., Spradling, A., Tsang, G., Wan, K., Whitelaw, K., Kimmel, B., Celniker, S., Rubin, G.M. (1999). An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster. The Adh region. Genetics 153(1): 179--219. (Export to RIS)|
|Publication Type||Research paper|
|PubMed Abstract||A contiguous sequence of nearly 3 Mb from the genome of Drosophila melanogaster has been sequenced from a series of overlapping P1 and BAC clones. This region covers 69 chromosome polytene bands on chromosome arm 2L, including the genetically well-characterized "Adh region." A computational analysis of the sequence predicts 218 protein-coding genes, 11 tRNAs, and 17 transposable element sequences. At least 38 of the protein-coding genes are arranged in clusters of from 2 to 6 closely related genes, suggesting extensive tandem duplication. The gene density is one protein-coding gene every 13 kb; the transposable element density is one element every 171 kb. Of 73 genes in this region identified by genetic analysis, 49 have been located on the sequence; P-element insertions have been mapped to 43 genes. Ninety-five (44%) of the known and predicted genes match a Drosophila EST, and 144 (66%) have clear similarities to proteins in other organisms. Genes known to have mutant phenotypes are more likely to be represented in cDNA libraries, and far more likely to have products similar to proteins of other organisms, than are genes with no known mutant phenotype. Over 650 chromosome aberration breakpoints map to this chromosome region, and their nonrandom distribution on the genetic map reflects variation in gene spacing on the DNA. This is the first large-scale analysis of the genome of D. melanogaster at the sequence level. In addition to the direct results obtained, this analysis has allowed us to develop and test methods that will be needed to interpret the complete sequence of the genome of this species. Before beginning a Hunt, it is wise to ask someone what you are looking for before you begin looking for it. Milne 1926|
|Supplementary material||Table S1. P-element insertions in the Adh region. [FBrf0112150]
Table S2. Genes, known and predicted, that have been determined on the sequence of the Adh region. All genes are protein coding, unless indicated tscan in the prediction column, these are tRNAs. [FBrf0112151]
Table S3. A classified summary of the inferred function of the products of 91 genes in the Adh region. [FBrf0112152]
|Review||Genomics. The millennium flies in.
Burtis and Hawley, 1999, Nature 401(6749): 125, 127 [FBrf0111336]
|Erratum||An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster. The Adh region.
Ashburner et al., 1999, Genetics 153(3): 1491 [FBrf0125014]
|Personal communication to FlyBase||Adh.
Ashburner et al., 1999.10.12, Adh. [FBrf0112153]
What does this section display?
What does this section not display?
This section does not currently display links that were removed or gene model changes.
|All updates||Click here to see a list of all updates to this record from FB2010_08 and on.|
|Language of Publication||English|
|Additional Languages of Abstract|
|Also Published As|
|Data from Reference|
|Natural transposons (10)|