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Citation
Ashburner, M., Misra, S., Roote, J., Lewis, S.E., Blazej, R., Davis, T., Doyle, C., Galle, R., George, R., Harris, N., Hartzell, G., Harvey, D., Hong, L., Houston, K., Hoskins, R., Johnson, G., Martin, C., Moshrefi, A., Palazzolo, M., Reese, M.G., Spradling, A., Tsang, G., Wan, K., Whitelaw, K., Kimmel, B., Celniker, S., Rubin, G.M. (1999). An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster. The Adh region.  Genetics 153(1): 179--219.
FlyBase ID
FBrf0111326
Publication Type
Research paper
Abstract
A contiguous sequence of nearly 3 Mb from the genome of Drosophila melanogaster has been sequenced from a series of overlapping P1 and BAC clones. This region covers 69 chromosome polytene bands on chromosome arm 2L, including the genetically well-characterized "Adh region." A computational analysis of the sequence predicts 218 protein-coding genes, 11 tRNAs, and 17 transposable element sequences. At least 38 of the protein-coding genes are arranged in clusters of from 2 to 6 closely related genes, suggesting extensive tandem duplication. The gene density is one protein-coding gene every 13 kb; the transposable element density is one element every 171 kb. Of 73 genes in this region identified by genetic analysis, 49 have been located on the sequence; P-element insertions have been mapped to 43 genes. Ninety-five (44%) of the known and predicted genes match a Drosophila EST, and 144 (66%) have clear similarities to proteins in other organisms. Genes known to have mutant phenotypes are more likely to be represented in cDNA libraries, and far more likely to have products similar to proteins of other organisms, than are genes with no known mutant phenotype. Over 650 chromosome aberration breakpoints map to this chromosome region, and their nonrandom distribution on the genetic map reflects variation in gene spacing on the DNA. This is the first large-scale analysis of the genome of D. melanogaster at the sequence level. In addition to the direct results obtained, this analysis has allowed us to develop and test methods that will be needed to interpret the complete sequence of the genome of this species. Before beginning a Hunt, it is wise to ask someone what you are looking for before you begin looking for it. Milne 1926
PubMed ID
PubMed Central ID
PMC1460734 (PMC) (EuropePMC)
DOI
Related Publication(s)
Review
Genomics. The millennium flies in.
Burtis and Hawley, 1999, Nature 401(6749): 125, 127 [FBrf0111336]
Erratum
An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster. The Adh region.
Ashburner et al., 1999, Genetics 153(3): 1491 [FBrf0125014]
Personal communication to FlyBase
Adh.
Ashburner et al., 1999.10.12, Adh. [FBrf0112153]
Associated Information
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Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Aberrations (61)
    Alleles (102)
    Genes (290)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Natural transposons (10)
    Insertions (59)
    Transgenic Constructs (3)