Reference Report

Reference
Citation	Ashburner, M., Misra, S., Roote, J., Lewis, S.E., Blazej, R., Davis, T., Doyle, C., Galle, R., George, R., Harris, N., Hartzell, G., Harvey, D., Hong, L., Houston, K., Hoskins, R., Johnson, G., Martin, C., Moshrefi, A., Palazzolo, M., Reese, M.G., Spradling, A., Tsang, G., Wan, K., Whitelaw, K., Kimmel, B., Celniker, S., Rubin, G.M. (1999). An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster. The Adh region. Genetics 153(1): 179--219. (Export to RIS)
FlyBase ID	FBrf0111326
Publication Type	Research paper
PubMed ID	10471707
PubMed Abstract	A contiguous sequence of nearly 3 Mb from the genome of Drosophila melanogaster has been sequenced from a series of overlapping P1 and BAC clones. This region covers 69 chromosome polytene bands on chromosome arm 2L, including the genetically well-characterized "Adh region." A computational analysis of the sequence predicts 218 protein-coding genes, 11 tRNAs, and 17 transposable element sequences. At least 38 of the protein-coding genes are arranged in clusters of from 2 to 6 closely related genes, suggesting extensive tandem duplication. The gene density is one protein-coding gene every 13 kb; the transposable element density is one element every 171 kb. Of 73 genes in this region identified by genetic analysis, 49 have been located on the sequence; P-element insertions have been mapped to 43 genes. Ninety-five (44%) of the known and predicted genes match a Drosophila EST, and 144 (66%) have clear similarities to proteins in other organisms. Genes known to have mutant phenotypes are more likely to be represented in cDNA libraries, and far more likely to have products similar to proteins of other organisms, than are genes with no known mutant phenotype. Over 650 chromosome aberration breakpoints map to this chromosome region, and their nonrandom distribution on the genetic map reflects variation in gene spacing on the DNA. This is the first large-scale analysis of the genome of D. melanogaster at the sequence level. In addition to the direct results obtained, this analysis has allowed us to develop and test methods that will be needed to interpret the complete sequence of the genome of this species. Before beginning a Hunt, it is wise to ask someone what you are looking for before you begin looking for it. Milne 1926
DOI
Related Publication(s)
Supplementary material	Table S1. P-element insertions in the Adh region. [FBrf0112150] Table S2. Genes, known and predicted, that have been determined on the sequence of the Adh region. All genes are protein coding, unless indicated tscan in the prediction column, these are tRNAs. [FBrf0112151] Table S3. A classified summary of the inferred function of the products of 91 genes in the Adh region. [FBrf0112152]
Review	Genomics. The millennium flies in. Burtis and Hawley, 1999, Nature 401(6749): 125, 127 [FBrf0111336]
Erratum	An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster. The Adh region. Ashburner et al., 1999, Genetics 153(3): 1491 [FBrf0125014]
Personal communication to FlyBase	Adh. Ashburner et al., 1999.10.12, Adh. [FBrf0112153]
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (61)
	Df(2L)64j Df(2L)A47 Df(2L)A72 Df(2L)A178 Df(2L)A263 Df(2L)A400 Df(2L)Adh-nBR55 Df(2L)Antp^Ctxrv1 Df(2L)b74c6 Df(2L)b84a7 Df(2L)b84h1 Df(2L)b85f1A Df(2L)b88f32 Df(2L)b88h49 Df(2L)el20 Df(2L)el80f1 Df(2L)fn3 Df(2L)H60-3 Df(2L)III18 Df(2L)k08712-rv21 Df(2L)osp18 Df(2L)osp29 Df(2L)osp38 Df(2L)RA5 Df(2L)rd9 Df(2L)RM5 Df(2L)RN2 Df(2L)Sco^rv25 Df(2L)TE35B-7 Df(2L)TE35B-9 Df(2L)TE35B-407 Df(2L)TE35D-2 Df(2L)TE35D-3 Df(2L)TE35D-5 Df(2L)TE35D-14 Df(2L)TE35D-17 Df(2L)TE35D-19 Df(2L)TE35D-22 Dp(2;3)osp³ In(2L)75c In(2L)b82a1 In(2L)C163.41 In(2L)dpp^d36 In(2L)dpp^s22 In(2L)el18 In(2LR)DTD121 T(2;3)6r28 T(2;3)b89e12 T(2;3)b^79d6 T(2;3)G16 T(2;3)H68 T(2;4)DTD22 T(2;4)TE35B-101 T(Y;2)b8 Tp(1;2)TE34Ca Tp(1;2)TE35B Tp(1;2)TE35BC Tp(1;2)TE35D Tp(2;2)A446 Tp(3;2)Antp^Ctx Ts(2Lt;4Lt)TE35B-101+Ts(2Rt;4Rt)DTD22
Alleles (102)
	Ance^+t16.5 B4⁰⁵³³⁷ B4^EP2503 B4^k01405 b^79d6 beat-Ia^AM3 beat-Ia^C163 beat-Ia^tric-1 bgm^k09909 cact^k17003 CenG1A⁰⁶⁶⁴⁶ CenG1A^k10802 CG4455^k04216 CG15269⁰⁵⁴⁴¹ ck⁰⁷¹³⁰ ck^EP2051 ck^TE35BC crp⁰⁰²³² crp^EP721 crp^k07829 crp^RAR46 Cul-3⁰⁶⁴³⁰ CycE¹ CycE⁵ CycE⁰⁵²⁰⁶ CycE^k02602 Cyp303a1^unspecified dac^unspecified elA¹ elB^EP2039 elB^k07706 esg⁰⁷⁰⁸² esg^dgl fzy^EP1028 Gli^k09033 heix^k11403 heix^unspecified kuz¹ kuz² kuz³ kuz^k01403 kuz^k01405 l(2)35Bc^k08808 l(2)35Bd¹⁰⁴⁰⁸ l(2)35Bd^Ctx l(2)35Bg^k10011 l(2)35Df¹ l(2)35Df^HL58 l(2)35Df^k14423 l(2)k07245^k07245 l(3)06264⁰⁶²⁶⁴ lace³ lace^k05305 mol^k11524a mRpL4^k14608 ms(2)35Ci⁰²³¹⁶ NimC3^EP2418 NimC3^k00811 noc^EP2000 noc^TE35B osp^k13218 osp^rJ571 pburs^unspecified Pgant35A^+tGal PRL-1^03264b PRL-1^EP311 PRL-1^k09834 Rab14^k08712 rk^11P rk^unspecified RpII33^k05605 Smg5^EP2171 Smg5^RACE smi35A¹ smi35A^k06901 smi35A^k11509 smi35A^k16716 sna^Sco Sos^k05224 stc⁶ stc⁰⁵⁴⁴¹ Su(H)^k07904 tam^unspecified TM9SF4^k07245 twe¹ twe^+t10 twe^DTD22 twe^EP613 twe^k08310 twe^RN2 twe^unspecified vas³ vas^EP812 vas^k07233 vig^EP812 vig^k07233 wb^6r28 wb⁰⁹⁴³⁷ wb^DTD121 wb^H68 wb^k16716 wek^05271a
Constructs (3)
	P{CaGal} P{RACE} P{twe⁺10.0}
Genes (291)
	All Genes relating to this reference
Insertions (59)
	P{EP}B4^EP2503 P{EP}ck^EP2051 P{EP}crp^EP721 P{EP}elB^EP2039 P{EP}fzy^EP1028 P{EP}NimC3^EP2418 P{EP}noc^EP2000 P{EP}PRL-1^EP311 P{EP}Smg5^EP2171 P{EP}twe^EP613 P{EP}vig^EP812 P{lacW}bgm^k09909 P{lacW}cact^k17003 P{lacW}CenG1A^k10802 P{lacW}CG4455^k04216 P{lacW}crp^k07829 P{lacW}CycE^k02602 P{lacW}elB^k07706 P{lacW}Gli^k09033 P{lacW}heix^k11403 P{lacW}kuz^k01403 P{lacW}kuz^k01405 P{lacW}l(2)35Bc^k08808 P{lacW}l(2)35Bg^k10011 P{lacW}l(2)35Df^k14423 P{lacW}lace^k05305 P{lacW}mol^k11524a P{lacW}mRpL4^k14608 P{lacW}NimC3^k00811 P{lacW}osp^k13218 P{lacW}PRL-1^k09834 P{lacW}Rab14^k08712 P{lacW}RpII33^k05605 P{lacW}smi35A^k06901 P{lacW}smi35A^k11509 P{lacW}Sos^k05224 P{lacW}Su(H)^k07904 P{lacW}TM9SF4^k07245 P{lacW}twe^k08310 P{lacW}vas^k07233 P{lacW}wb^k16716 P{lArB}smi35A¹ P{PZ}09259 P{PZ}B4⁰⁵³³⁷ P{PZ}CenG1A⁰⁶⁶⁴⁶ P{PZ}ck⁰⁷¹³⁰ P{PZ}crp⁰⁰²³² P{PZ}Cul-3⁰⁶⁴³⁰ P{PZ}CycE⁰⁵²⁰⁶ P{PZ}esg⁰⁷⁰⁸² P{PZ}l(2)35Bd¹⁰⁴⁰⁸ P{PZ}l(3)06264⁰⁶²⁶⁴ P{PZ}ms(2)35Ci⁰²³¹⁶ P{PZ}osp^rJ571 P{PZ}PRL-1^03264b P{PZ}stc⁰⁵⁴⁴¹ P{PZ}wb⁰⁹⁴³⁷ P{PZ}wek^05271a P{wA^R}rk^11P
Natural transposons (10)
	297 blood copia Doc F-element G-element jockey mdg1 opus roo