Reference Report

Reference
Citation	Chen, G., Fernandez, J., Mische, S., Courey, A.J. (1999). A functional interaction between the histone deacetylase rpd3 and the corepressor groucho in Drosophila development. Genes Dev. 13(17): 2218--2230. (Export to RIS)
FlyBase ID	FBrf0111344
Publication Type	Research paper
PubMed ID	10485845
PubMed Abstract	The Drosophila gene groucho (gro) encodes a transcriptional corepressor that has critical roles in many development processes. In an effort to illuminate the mechanism of Gro-mediated repression, we have employed Gro as an affinity reagent to purify Gro-binding proteins from embryonic nuclear extracts. One of these proteins was found to be the histone deacetylase Rpd3. Protein-protein interaction assays suggest that Gro and Rpd3 form a complex in vivo and that they interact directly via the glycine/proline rich (GP) domain in Gro. Cell culture assays demonstrate that Rpd3 potentiates repression by the GP domain. Furthermore, experiments employing a histone deacetylase inhibitor, as well as a catalytically inactive form of Rpd3, imply that histone deacetylase activity is required for efficient Gro-mediated repression. Finally, mutations in gro and rpd3 have synergistic effects on embryonic lethality and pattern formation. These findings support the view that Gro mediates repression, at least in part, by the direct recruitment of the histone deacetylase Rpd3 to the template, where it can modulate local chromatin structure. They also provide evidence for a specific role of Rpd3 in early development.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Aberrations (2)
	Df(3L)10^H Df(3R)Espl22
Alleles (17)
	dl^Act5C.PS Ecol\lacZ^ftz.P gro^{1-719.Act5C.T:Scer\GAL4} gro^{121-194.Act5C.T:Scer\GAL4,T:Hsap\TP53} gro^{121-194.Act5C.T:Scer\GAL4} gro^{391-719.Act5C.T:Scer\GAL4} gro^E48 Ppyr\LUC^{Hsim\TK.dl.Scer\UAS} Rpd3^15-1 Rpd3⁰⁴⁵⁵⁶ Rpd3^{H196F.Act5C.T:Scer\GAL4} Rpd3^H196F.Act5C Rpd3^{WT.Act5C.T:Scer\GAL4} Rpd3^{WT.Act5C.T:Zzzz\FLAG} Scer\GAL4^{1-147.cCa.Act5C} Scer\GAL4^{TD.Act5C.T:Hsap\TP53} twi^Act5C.PS
Constructs (1)
	P{ftz-lacZ.ry⁺}
Genes (11)
	dl Ecol\lacZ gro His1 Ppyr\LUC Rpd3 Scer\GAL4 T:Hsap\TP53 T:Scer\GAL4 T:Zzzz\FLAG twi
Insertions (3)
	P{hsFLP}22 P{hsneo.ry⁺}Rpd3^15-1 P{PZ}Rpd3⁰⁴⁵⁵⁶